Activation of oral trigeminal neurons by fatty acids is dependent upon intracellular calcium.
Activation of oral trigeminal neurons by fatty acids is dependent upon intracellular calcium.
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DOI：
10.1007/s00424-012-1116-9
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发表时间：
2012-08
影响因子：
4.5
通讯作者：
Gilbertson, Timothy A.
中科院分区：
文献类型：
--
作者：
Yu, Tian;Shah, Bhavik P.;Hansen, Dane R.;Park-York, MieJung;Gilbertson, Timothy A.
饮食脂肪在口腔中的化学感受主要归因于传递脂肪质地特性的躯体感觉系统的激活。然而，脂肪酸，这被认为是代表脂肪味道的最接近的刺激，刺激大鼠三叉神经元的能力仍然是未知的。在这里，我们发现几种游离脂肪酸能够以不同的动力学激活三叉神经元。此外，多不饱和脂肪酸亚油酸（LA）通过增加细胞内钙浓度和产生去极化受体电位来激活三叉神经元。离子替代和药理学方法揭示，细胞内钙库耗竭是至关重要的LA诱导的信号在三叉神经元的一个子集。使用伪狂犬病病毒（PrV）作为活细胞示踪剂，我们确定了舌神经支配的三叉神经元的一个子集，对不同的脂肪酸子集作出反应。几个瞬时受体电位（TRP）通道标记在个别神经元的定量实时PCR验证，PrV标记的一个子集，但不是整个人口的舌神经支配的三叉神经元。我们进一步证实，LA诱导的细胞内钙升高是完全来自释放的钙存储从内质网在这个子集的舌神经支配的三叉神经神经元。
The chemoreception of dietary fat in the oral cavity has largely been attributed to activation of the somatosensory system that conveys the textural properties of fat. However, the ability of fatty acids, which are believed to represent the proximate stimulus for fat taste, to stimulate rat trigeminal neurons has remained unexplored. Here, we found that several free fatty acids are capable of activating trigeminal neurons with different kinetics. Further, a polyunsaturated fatty acid, linoleic acid (LA), activates trigeminal neurons by increasing intracellular calcium concentration and generating depolarizing receptor potentials. Ion substitution and pharmacological approaches reveal that intracellular calcium store depletion is crucial for LA-induced signaling in a subset of trigeminal neurons. Using pseudorabies virus (PrV) as a live cell tracer, we identified a subset of lingual nerve-innervated trigeminal neurons that respond to different subsets of fatty acids. Quantitative real-time PCR of several transient receptor potential (TRP) channel markers in individual neurons validated that PrV labeled a subset but not the entire population of lingual-innervated trigeminal neurons. We further confirmed that the LA-induced intracellular calcium rise is exclusively coming from the release of calcium stores from the endoplasmic reticulum in this subset of lingual nerve-innervated trigeminal neurons.
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影响因子：
7.8
作者：
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影响因子：
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作者：
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作者：
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通讯作者：
Enquist, L W
DOI：
10.1073/pnas.0810801105
发表时间：
2008-12-16
影响因子：
11.1
作者：
Bhattacharya, Martha R. C.;Bautista, Diana M.;Julius, David
通讯作者：
Julius, David