Frequency of Antibodies to Various Antigenic Determinants in Polytransfused Patients with Homozygous Thalassaemia in Greece
Frequency of Antibodies to Various Antigenic Determinants in Polytransfused Patients with Homozygous Thalassaemia in Greece
复制标题
希腊多输血纯合性地中海贫血患者中各种抗原决定簇的抗体频率
DOI：
10.1111/j.1423-0410.1971.tb00436.x
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发表时间：
1971
期刊：
影响因子：
2.7
通讯作者：
M. Adinolfi
中科院分区：
文献类型：
--
作者：
J. Economidou;M. Constantoulakis;O. Augoustaki;M. Adinolfi
对147例多输血的纯合子地中海贫血患者的血清进行了红细胞抗原抗体的检测;对79例患者的血清进行了血小板抗体检测;对上述样本中的60例进行了白色细胞、Gm、Inv和Ag抗原决定簇抗体的检测。每种抗体的频率和特异性与输血量、输血反应和临床参数（如脾切除）相关。这些患者年龄从3岁到24岁，在2到18年的时间里接受了10到287次输血;从所有病例中收集了输血前的血液样本。使用盐水凝集和抗球蛋白试验研究红细胞抗体的存在[1]。147例患者中有3例检测到抗K抗体，另1例检测到特异性不明的不完全温抗体。此外，9名Rh阴性儿童中有4名在未知数量的不相容输血后产生了抗Rhantigens抗体;所有4名患者均形成了抗D抗体; 1名患者还具有抗C抗体，另1名患者具有抗C抗体和抗E抗体。2例患者有抗I冷凝集素（滴度1：8）。通过EDTA凝集技术[21]检测由5-8名供体组成的小组中的白细胞凝集素。用微滴技术检测抗淋巴细胞的细胞毒抗体，
Sera from 147 polytransfused patients with homozygous thalas saemia were investigated for the presence of antibodies against red cell antigens; 79 sera were tested for platelet antibodies; 60 of the above samples were also investigated for the presence of antibodies against white cells, Gm, Inv and Ag antigenic determinants. The frequency and specificity of each type of antibody were correlated with the amount of transfused blood, transfusion reactions and clinical parameters, such as splenectomy. The patients, from 3-to 24-years-old, had received from 10 to 287 transfusions over a period ranging from 2 to 18 years; pre-transfusion blood samples were collected from all cases. The presence of red cell antibodies was investigated using agglutina tion in saline and anti-globulin tests [1]. Anti-K antibodies were de tected in 3 out of 147 patients; a further incomplete warm antibody of unidentified specificity was detected in another patient. Moreover, 4 Rh-negative children out of 9 developed antibodies against Rhantigens after an unknown number of incompatible blood trans fusions; all 4 patients formed anti-D; one also had anti-C and another anti-C and anti-E. Two patients had anti-I cold agglutinin (titre 1: 8). Leucoagglutinins were detected by the EDTA agglutination tech nique [21], against a panel formed by 5-8 donors. Cytotoxic anti bodies against lymphocytes were detected by the microdroplet tech-