Peritoneal macrophages of guinea pig possibly lack LTC4 synthetase.
Peritoneal macrophages of guinea pig possibly lack LTC4 synthetase.
复制标题
豚鼠腹腔巨噬细胞可能缺乏LTC4合成酶。
DOI：
10.1016/s0006-291x(85)80119-4
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
N. Shigematsu
中科院分区：
文献类型：
--
作者：
M. Abe;Y. Kawazoe;H. Tsunematsu;N. Shigematsu
a23187体外刺激小鼠和大鼠腹膜细胞和贴壁细胞合成ltc4和ltb4。另一方面，豚鼠的腹膜细胞和贴壁细胞均未产生LTC4、D4和E4，但LTB4的含量较低。同时添加10 μM a.a可促进该物种ltb4的产生。从这三个物种的腹膜细胞中提取的酶溶液能够在谷胱甘肽存在的情况下将DNCB转化为有色产物这些效力依次是；豚鼠b>小鼠b>大鼠。另一方面，在谷胱甘肽存在下，lta4转化为ltc4的效力依次为：小鼠>大鼠≈0天竺鼠这些结果表明，豚鼠巨噬细胞缺乏“ltc4合成酶”，并且该酶与通常的GSH s转移酶不同。
Peritoneal cells and adherent cells of mice and rats synthesized LTC4and LTB4when stimulated with A23187in vitro. On the other hand, neither peritoneal cells nor adherent cells of guinea pigs generated LTC4, D4, and E4, but did the lower amounts of LTB4. Only generation of LTB4was potentiated by simultaneous addition of 10 μM A.A. in this species. Enzyme solutions which were extracted from peritoneal cells of these three species were capable of converting DNCB to a colored product in the presence of glutathione and then these potencies were in the following order; guinea pig > mouse > rat. On the other hand, the potencies of converting LTA4to LTC4in the presence of glutathione were in the following order; mouse > rat ≫ guinea pig ≈ 0. These results suggest that macrophages of guinea pigs lack “LTC4synthetase” and also this enzyme is different from usual GSH S-transferases.