In vivo imaging of cGMP in plants.

In vivo imaging of cGMP in plants.
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植物中 cGMP 的体内成像。

DOI:
10.1007/978-1-62703-441-8_5
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发表时间:
2013
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Isner JC
Isner JC
中科院分区:
--
文献类型:
--
作者:
Isner JC

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环核苷酸3′,5′-环鸟酰单磷酸(cGMP)参与了重要植物过程的调控。为了进一步揭示其生理作用,准确记录cGMP浓度的动态变化是必要的。用于“实时成像”的基于生物分子的荧光传感器是理想的,因为它们具有高特异性,在生物相关浓度范围内的灵敏度,高空间和动态分辨率,并且使用此类传感器进行测量是非破坏性的。在本章中,我们描述了cGMP FlincG传感器在植物材料中的使用,这些植物材料可以瞬时或稳定地表达该传感器。
The cyclic nucleotide 3′,5′-cyclic guanyl monophosphate (cGMP) has been implicated in the regulation of important plant processes. To unravel its physiological role further, accurate recording of dynamic changes in cGMP concentration is necessary. Fluorescent sensors based on biological molecules for “live imaging” are ideal for this since they have high specificity, a sensitivity that is in the range of biologically relevant concentrations, high spatial and dynamic resolution, and measurements with such sensors are nondestructive. In this chapter we describe the use of the cGMP FlincG sensor in plant materials that either transiently or stably express this sensor.
DOI: 10.1111/j.1365-313x.2010.04418.x
发表时间: 2011-01-01
期刊: PLANT JOURNAL
影响因子: 7.2
作者:
Isner, Jean-Charles;Maathuis, Frans J. M.
通讯作者: Maathuis, Frans J. M.
DOI: 10.1111/j.1365-313x.1994.00421.x
发表时间: 1994-03-01
期刊: PLANT JOURNAL
影响因子: 7.2
作者:
ABEL, S;THEOLOGIS, A
通讯作者: THEOLOGIS, A