Fast and accurate peanut allergen detection with nanobead enhanced optical fiber SPR biosensor
Fast and accurate peanut allergen detection with nanobead enhanced optical fiber SPR biosensor
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DOI：
10.1016/j.talanta.2010.11.032
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发表时间：
2011-02-15
期刊：
影响因子：
6.1
通讯作者：
Lammertyn, J.
中科院分区：
文献类型：
--
作者：
Pollet, J.;Delport, F.;Lammertyn, J.
本文首次报道了一种纳米珠增强型光纤SPR生物传感器。我们评估了该系统的快速和准确的检测复杂的食品基质中的花生过敏原的生物测定。三种方法的免疫测定检测阿糖胞苷h1花生过敏原的巧克力糖果条进行了比较;无标记的测定，二抗夹心测定和纳米珠增强测定。虽然无标记检测是最方便的，我们的研究结果表明，功能化的纳米珠可以提供一个完善的解决方案，以提高光纤SPR检测限。通过应用磁铁矿纳米颗粒作为二级标记，SPR生物测定的检测限为Ara H1提高了两个数量级，从9到0.09 μ g/mL。纳米颗粒的超顺磁性特性确保了易于处理。SPR光纤可再生，一根光纤可重复使用35次而不损失灵敏度。结果以市售多克隆ELISA试剂盒为基准。发现用ELISA获得的Ara h1浓度与用SPR纤维测定法测量的浓度之间具有良好的相关性。此外，与最快的ELISA方案相比，使用SPR光纤，我们可以以两倍的速度测量样品。由于浸染条纤维不需要可能堵塞的微通道，因此可以避免耗时的冲洗步骤。所提出的传感器的线性动态范围为0.1和2 μ g/mL之间，这是大大大于ELISA基准。(C)2010 Elsevier B. V.保留所有权利。
This paper is the first report of a fiber optic SPR biosensor with nanobead signal enhancement. We evaluated the system with a bioassay for the fast and accurate detection of peanut allergens in complex food matrices. Three approaches of an immunoassay to detect Ara h1 peanut allergens in chocolate candy bars were compared; a label-free assay, a secondary antibody sandwich assay and a nanobead enhanced assay. Although label-free detection is the most convenient, our results illustrate that functionalized nanobeads can offer a refined solution to improve the fiber SPR detection limit. By applying magnetite nanoparticles as a secondary label, the detection limit of the SPR bioassay for Ara h1 was improved by two orders of magnitude from 9 to 0.09 mu g/mL. The super paramagnetic character of the nanoparticles ensured easy handling. The SPR fibers could be regenerated easily and one fiber could be reused for up to 35 times without loss of sensitivity. The results were benchmarked against a commercially available polyclonal ELISA kit. An excellent correlation was found between the Ara h1 concentrations obtained with the ELISA and the concentrations measured with the SPR fiber assay. In addition, with the SPR fiber we could measure the samples twice as fast as compared to the fastest ELISA protocol. Since the dipstick fiber has no need for microchannels that can become clogged, time consuming rinsing step could be avoided. The linear dynamic range of the presented sensor was between 0.1 and 2 mu g/mL, which is considerably larger than the ELISA benchmark. (C) 2010 Elsevier B.V. All rights reserved.