Comparative Analyses of Dormancy-associated MADS-box Genes, PpDAM5 and PpDAM6, in Low- and High-chill Peaches (Prunus persica L.)
Comparative Analyses of Dormancy-associated MADS-box Genes, PpDAM5 and PpDAM6, in Low- and High-chill Peaches (Prunus persica L.)
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DOI：
10.2503/jjshs1.80.276
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发表时间：
2011-07
影响因子：
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通讯作者：
H. Yamane;R. Tao;Tomomi Ooka;Hiroaki Jotatsu;Ryuta Sasaki;K. Yonemori
中科院分区：
文献类型：
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作者：
H. Yamane;R. Tao;Tomomi Ooka;Hiroaki Jotatsu;Ryuta Sasaki;K. Yonemori
本研究探讨了PpDAM 5和PpDAM 6的季节性表达的调节，两个桃（桃）休眠相关的MADS盒基因，在有关的内休眠和发展的侧营养芽和花芽的低，高低温桃品种。PpDAM 5和PpDAM 6最初被发现是在桃的EVERGROWING（EVG）基因座处的拟南芥SVP/AGL 24的同源物，并且最近被证明参与侧芽内休眠。在这项研究中的季节性表达分析表明，PpDAM 5和PpDAM 6的转录水平在侧营养芽的低和高的寒冷品种在该领域与芽爆裂百分比在强迫条件下确定呈负相关。它们的转录水平与花器官膨大率呈负相关。这些结果表明，不同的季节性表达模式的PpDAM 5和PpDAM 6与一个不同的低温需求的芽断裂和开花的低和高的寒冷品种。PpDAM 5和PpDAM 6基因组结构的表征揭示了在低温桃中PpDAM 5和PpDAM 6的第一内含子中存在大的插入。基因组结构的改变进行了讨论，相对于低冷字符。
This study investigated the regulation of the seasonal expression of PpDAM5 and PpDAM6, two of the six peach (Prunus persica) dormancy-associated MADS-box genes, in relation to the endodormancy and development of lateral vegetative and flower buds of low- and high-chill peach cultivars. PpDAM5 and PpDAM6 were originally found as homologs of Arabidopsis SVP/AGL24 at the EVERGROWING (EVG) locus of peach and have been recently shown to be involved in lateral bud endodormancy. Seasonal expression analyses in this study indicated that PpDAM5 and PpDAM6 transcript levels in lateral vegetative buds of both low- and high-chill cultivars in the field negatively correlated with bud burst percentages determined under forcing conditions. Negative correlation was also found between their transcript levels and the flower organ enlargement rate. These results suggest that distinct seasonal expression patterns of PpDAM5 and PpDAM6 are correlated with a distinct chilling requirement for bud break and flowering of low- and high-chill cultivars. Characterization of the genomic structure of PpDAM5 and PpDAM6 revealed the presence of large insertions in the first introns of both PpDAM5 and PpDAM6 in low-chill peach. Alteration of the genomic structure is discussed with respect to the low-chill character.