The anatomical pathways for antennal sensory information in the central nervous system of the cricket, Gryllus bimaculatus
The anatomical pathways for antennal sensory information in the central nervous system of the cricket, Gryllus bimaculatus
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DOI：
10.1007/s10158-012-0137-6
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发表时间：
2012-06
影响因子：
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通讯作者：
Atsushi Yoritsune;H. Aonuma
中科院分区：
文献类型：
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作者：
Atsushi Yoritsune;H. Aonuma
触角是检测蟋蟀化学和机械感觉线索的主要器官之一。人们对蟋蟀如何在大脑中处理和整合不同形式的信息知之甚少。因此，我们使用了许多不同的解剖技术来了解从触角感觉神经元延伸到大脑中心的神经通路。我们利用触角神经的顺行染料填充确定了七个触角感觉束（指定为 T1-7）。 T1-T4 束投射到触角叶 (AL)，T5 和 T6 束伸入中脑或食管下神经节的背侧区域，最后，T7 束终止于鞭毛传入 (VFA) 的腹侧区域。通过分析 AL 的自发荧光图像，我们根据形状、相对位置和大小鉴定了 49 个性别同构的肾小球。根据我们的感觉束数据，我们将肾小球分为四个不同的组之一。然后，我们三维重建 AL（肾小球）和 VFA（层）的内部结构。接下来，在原大脑中，我们确定了 AL 和 VFA 的纤维束及其末端。我们发现 10 个区域源自 AL，而至少有 8 个区域源自 VFA。 AL 的一些路段与 VFA 的路段共享路线，但终点区域是隔离的。现在，我们对板球触角信息的通路有了更好的解剖学了解。
Antennae are one of the major organs to detect chemo- and mechanosensory cue in crickets. Little is known how crickets process and integrate different modality of information in the brain. We thus used a number of different anatomical techniques to gain an understanding of the neural pathways extending from the antennal sensory neurons up to centers in the brain. We identified seven antennal sensory tracts (assigned as T1–7) utilizing anterograde dye filling from the antennal nerve. Tracts T1–T4 project into the antennal lobe (AL), while tracts T5 and T6 course into the dorsal region of the deutocerebrum or the suboesophageal ganglion, and finally, tract T7 terminates in the ventral area of flagellar afferent (VFA). By analyzing autofluorescence images of the AL, we identified 49 sexually isomorphic glomeruli on the basis of shape, relative position and size. On the basis of our sensory-tract data, we assigned the glomeruli into one of four separate groups. We then three-dimensionally reconstructed the internal structures in the AL (glomeruli) and the VFA (layers). Next in the protocerebrum, we identified both the tracts and their terminations from the AL and VFA. We found that 10 tracts originate in the AL, whereas there are at least eight tracts from the VFA. Several tracts from the AL share their routes with those from the VFA, but their termination areas are segregated. We now have a better anatomical understanding of the pathways for the antennal information in cricket.