The complete mitochondrial genomes of four cockroaches (Insecta: Blattodea) and phylogenetic analyses within cockroaches
The complete mitochondrial genomes of four cockroaches (Insecta: Blattodea) and phylogenetic analyses within cockroaches
复制标题
四种蟑螂（昆虫纲：蜚蠊总科）的完整线粒体基因组和蟑螂内部的系统发育分析
DOI：
10.1016/j.gene.2016.03.057
复制
发表时间：
2016-07-15
期刊：
影响因子：
3.5
通讯作者：
Zhang, Jia-Yong
中科院分区：
文献类型：
--
作者：
Cheng, Xue-Fang;Zhang, Le-Ping;Zhang, Jia-Yong
为了进一步了解蟑螂有丝分裂组的特征，重建蟑螂的系统发育关系，测定了蟑螂(昆虫门：Blattodea)的3个完整线粒体基因组(Gromphadorhina portentosa、Panlora nivea、Blaptica dubia)和1个Blattidae(Insecta：Blattodea)的完整线粒体基因组序列。这4个蟑螂基因组的基因序列和方向与已知的蟑螂mt基因组相似，包含13个蛋白质编码基因(PCGs)、2个核糖体RNA(RRNA)基因、22个转移RNA(TRNA)基因和一个控制区。Blattodea的mt基因组具有高A+T组成(70.7%-74.3%)和以TAA终止密码子为主的特点。在已知蟑螂中，紫夜蛾线粒体全基因组、PCGs和总tRNAs的AT含量最低。在trnQ和trnM之间存在71个碱基的基因间隔区是B.dubia特有的特征，但在其他蟑螂中没有，这可以用复制/随机丢失模型来解释。基于13个PCGs基因的核苷酸和氨基酸序列，采用邻接法(NJ)、最大简约法(MP)、最大似然法(ML)和贝叶斯推理法(BI)重建了蟑螂的系统发育关系。所有的系统发育分析都一致地将等翅目列为隐翅虫科的姊妹群。叶蝉科和叶蝉科(叶蝉总科)是蝗科的姊妹种。在核苷酸和氨基酸数据集的NJ和MP分析，以及氨基酸数据集的ML和BI分析中，Corydiidae是所有剩余蟑螂物种的姊妹林地。(C)2016爱思唯尔B.V.保留所有权利。
Three complete mitochondrial genomes of Blaberidae (Insecta: Blattodea) (Gromphadorhina portentosa, Panchlora nivea, Blaptica dubia) and one complete mt genome of Blattidae (Insecta: Blattodea) (Shelfordella lateralis) were sequenced to further understand the characteristics of cockroach mitogenomes and reconstruct the phylogenetic relationship of Blattodea. The gene order and orientation of these four cockroach genomes were similar to known cockroach mt genomes, and contained 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and one control region. The mt genomes of Blattodea exhibited a characteristics of a high A + T composition (70.7%-74.3%) and dominant usage of the TAA stop codon. The AT content of the whole mt genome, PCGs and total tRNAs in G. portentosa was the lowest in known cockroaches. The presence of a 71-bp intergenic spacer region between trnQ and trnM was a unique feature in B. dubia, but absent in other cockroaches, which can be explained by the duplication/random loss model. Based on the nucleotide and amino acid datasets of the 13 PCGs genes, neighbor-joining (NJ), maximum parsimony (MP), maximum likelihood (ML) and bayesian inference (BI) analyses were used to rebuild the phylogenetic relationship of cockroaches. All phylogenetic analyses consistently placed Isoptera as the sister cluster to Cryptocercidae of Blattodea. Ectobiidae and Blaberidae (Blaberoidea) formed a sister Glade to Blattidae. Corydiidae is a sister Glade of all the remaining cockroach species with a high value in NJ and MP analyses of nucleotide and amino acid datasets, and ML and BI analyses of the amino acid dataset. (C) 2016 Elsevier B.V. All rights reserved.