Cloning and characterization of a novel dehydrin gene, SiDhn2, from Saussurea involucrata Kar. et Kir.
Cloning and characterization of a novel dehydrin gene, SiDhn2, from Saussurea involucrata Kar. et Kir.
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来自雪莲的新型脱水素基因 SiDhn2 的克隆和表征。
DOI：
10.1007/s11103-013-0164-7
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发表时间：
2014-04-01
影响因子：
5.1
通讯作者：
Zhu, Jian-Bo
中科院分区：
文献类型：
--
作者：
Qiu, Honglin;Zhang, Linhua;Zhu, Jian-Bo
天山雪莲Et Kir.是一种耐寒的双子叶植物，能够耐受严重的非生物胁迫条件。在之前的研究中，我们创建了一个cdna文库，以确定哪些因素与天山雪莲的冷驯化反应有关。在此基础上，通过RT-PCR获得了类脱水蛋白基因(SiDhn2)的全长cDNA。本研究对SiDhn2基因进行了特征分析。SiDhn2基因全长693个碱基，含有一个345个碱基的开放阅读框，编码115个氨基酸的蛋白质。推导的氨基酸序列比对表明，SiDhn2与两个油菜脱水蛋白有55%的同源性。利用根癌农杆菌将RD29A：SiDhn2和35S：SiDhn2转化烟草，研究转基因植株的萌发和抗寒性和抗旱性。RD29A：SiDhn2转基因植株表现出比35S：SiDhn2转基因植株和野生型植株更强的抗寒性和抗旱性。本研究表明，SiDhn2具有抗寒性和抗旱性，可能是作物提高抗逆性遗传改良的候选抗性基因。
Saussurea involucrata Kar. et Kir. is a hardy dicotyledonous plant capable of tolerating severe abiotic stress conditions. In a previous study, we created a cDNA library to determine what factors are associated with the cold acclimation response in S. involucrata. From this, a full-length cDNA of a dehydrin-like gene (SiDhn2) was obtained by RT-PCR. The SiDhn2 gene was characterized in this study. The full-length SiDhn2 cDNA comprised 693 bp containing an open reading frame of 345 bp specifying a protein of 115 amino acids. An alignment of the deduced amino acid sequence showed that SiDhn2 shared 55 % identity with two Brassica dehydrins. Agrobacterium tumefaciens was used to transform RD29A:SiDhn2 and 35S:SiDhn2 constructs into tobacco to investigate the germination and resistance to freezing and drought stress of transgenic plants. The RD29A:SiDhn2 transgenic plants showed greater resistance to freezing and drought stress than 35S:SiDhn2 transgenic plants or the wild-type. This study demonstrates that SiDhn2 confers cold hardiness and drought resistance, and may be a candidate resistance gene for genetic improvement of crops to increase stress resistance.