Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice
Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice
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DOI：
10.1042/bsr20170695
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发表时间：
2017-08-31
影响因子：
4
通讯作者：
Liu, Chun-Min
中科院分区：
文献类型：
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作者：
Zeng, Kun;Feng, Qi-Gao;Liu, Chun-Min
本研究以NAD(+)依赖的组蛋白去乙酰化酶sirtlin 1(SIRT1)为靶点，探讨miR-211对糖尿病白内障小鼠晶状体上皮细胞增殖和凋亡的影响。健康雄性小鼠随机分为正常白内障组和糖尿病白内障组。检测血糖、晶状体混浊度和细胞凋亡率。将晶状体上皮细胞分为正常组、空白对照组、阴性对照组、miR-211模拟物组、miR-211抑制剂组、siRNA-SIRT1组和miR-211抑制剂+siRNA-SIRT1组。检测各组MIR-211、Bcl2、Bax、P53、SIRT1的表达。用四甲基偶氮唑盐比色法和流式细胞仪检测细胞增殖、周期和细胞凋亡率。根据荧光素酶系统，MIR-211可以与SIRT1特异性结合。正常小鼠SIRT1蛋白呈强阳性，糖尿病白内障小鼠呈弱阳性。糖尿病白内障小鼠的细胞凋亡指数高于正常小鼠。与正常小鼠相比，糖尿病白内障小鼠的miR-211、Bax和P53的表达增加，而Bcl2和SIRT1的表达降低。与空白组和NC组相比，miR-211模拟物组和siRNA-SIRT1组miR-211、Bax和P53表达增加，而Bcl2和SIRT1表达降低，细胞增殖减少，凋亡率增加，而miR-211抑制剂组的结果与此相反。MIR-211可通过靶向SIRT1促进糖尿病白内障小鼠晶状体上皮细胞的凋亡和抑制其增殖。
Our study aimed at exploring the effects of miR-211 on the proliferation and apoptosis of lens epithelial cells in diabetic cataract mice by targetting NAD(+)-dependent histone deacetylase sirtulin 1 (SIRT1). Healthy male mice were assigned into normal and diabetic cataract groups. Blood glucose, lens turbidity, and apoptosis were measured. Lens epithelial cells were classified into the normal, blank, negative control (NC), miR-211 mimics, miR-211 inhibitors, siRNA-SIRT1, and miR-211 inhibitors + siRNA-SIRT1 groups. MiR-211, Bcl-2, Bax, p53, and SIRT1 expressions of each group were detected. Cell proliferation, cycle and apoptosis were tested by MTT assay and flow cytometry. MiR-211 can specifically bind to SIRT1 according to the luciferase system. SIRT1 protein concentration was strongly positive in normal mice and weakly positive in diabetic cataract mice. Apoptosis index of diabetic cataract mice was higher than the normal mice. Compared with normal mice, the expressions of miR-211, Bax, and p53 increased in diabetic cataract mice, while the Bcl-2 and SIRT1 expressions decreased. In comparison with the blank and NC groups, the expressions of miR-211, Bax, and p53 increased, while Bcl-2 and SIRT1 expressions decreased, and the proliferation decreased and apoptosis rate increased in the miR-211 mimics and siRNA-SIRT1 groups; the results were contradicting for the miR-211 inhibitor group. MiR-211 could promote apoptosis and inhibit proliferation of lens epithelial cells in diabetic cataract mice by targetting SIRT1.