Vibrio coralliilyticus sp nov., a temperature-dependent pathogen of the coral Pocillopora damicornis
Vibrio coralliilyticus sp nov., a temperature-dependent pathogen of the coral Pocillopora damicornis
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DOI：
10.1099/ijs.0.02402-0
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发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Rosenberg, E
中科院分区：
文献类型：
--
作者：
Ben-Haim, Y;Thompson, FL;Rosenberg, E
通过16 S rDNA序列测定、DNA-DNA杂交（G + C含量为45（.）6mol%）、AFLP和GTG（5）-PCR基因组指纹图谱和表型特性，包括细胞脂肪酸谱。主要脂肪酸为16：0和18：1 ω 7 c。新的珊瑚致病菌被命名为Vibrio coralliilyticus sp. nov.。除了分离自印度洋的菌株YB 1（T）之外，还分离出了另外5个溶珊瑚弧菌菌株，其中3个来自红海的患病P. damicornis，1个来自患病牡蛎幼虫（肯特，英国），1个来自双壳类幼虫（巴西）。6株溶珊瑚弧菌的基因型和表型相似性较高，均对珊瑚角单胞菌具有致病性。最接近的系统发育邻居是Vibrio tubiashii，Vibrio nereis和Vibrio shilonii。
Vibrio sp. YB1(T) (=ATCC BAA-450(T) =LMG 20984 T), the aetiological agent of tissue lysis of the coral Pocillopora damicornis, was characterized as a novel Vibrio species on the basis of 16S rDNA sequence, DNA-DNA hybridization data (G + C content is 45(.)6 mol%), AFLP and GTG(5)-PCR genomic fingerprinting patterns and phenotypic properties, including the cellular fatty acid profile. The predominant fatty acids were 16:0 and 18:1omega7c. The name Vibrio coralliilyticus sp. nov. is proposed for the novel coral-pathogenic species. In addition to strain YB1(T), which was isolated from the Indian Ocean, five additional strains of V. coralliilyticus have been isolated, three from diseased P. damicornis in the Red Sea, one from diseased oyster larvae (Kent, UK) and one from bivalve larvae (Brazil). The six V. coralliilyticus strains showed high genotypic and phenotypic similarities and all were pathogenic to P. damicornis. The closest phylogenetic neighbours to V. coralliilyticus are Vibrio tubiashii, Vibrio nereis and Vibrio shilonii.