Dietary Addition of Sodium Butyrate Contributes to Attenuated Feeding-Induced Hepatocyte Apoptosis in Dairy Goats
Dietary Addition of Sodium Butyrate Contributes to Attenuated Feeding-Induced Hepatocyte Apoptosis in Dairy Goats
复制标题
日粮中添加丁酸钠有助于减弱奶山羊饲养诱导的肝细胞凋亡。
DOI：
10.1021/acs.jafc.8b03526
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发表时间：
2018-09-26
影响因子：
6.1
通讯作者：
Shen, Xiangzhen
中科院分区：
文献类型：
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作者：
Chang, Guangjun;Liu, Xinxin;Shen, Xiangzhen
本研究旨在揭示饮食诱导肝细胞凋亡的机制。将18只泌乳中期山羊随机分为3组：低精料组（LC）、高精料组（HC）和丁酸钠（SB）添加组（SHC）。10周后，HC饮食成功地诱导亚急性瘤胃酸中毒（SARA），这增加了脂多糖（LPS）和细胞因子的浓度以及与炎症和凋亡相关的基因和蛋白的表达。在HC饲料中添加SB显著降低了这些参数的水平。HC组Bcl 2 mRNA和蛋白表达低于LC组和SHC组。此外，HC饮食减少了半胱天冬酶3和8启动子甲基化的百分比相比，那些喂食LC饮食的山羊，而SHC饮食部分恢复甲基化率，以减少半胱天冬酶3和8的表达。总的来说，HC饮食诱导的SARA通过激活外源性凋亡途径引起肝细胞凋亡，而SB饮食添加抑制炎症反应和减弱肝细胞凋亡。DNA甲基化对哺乳期山羊肝脏中关键凋亡基因的表达有调控作用。
The present study aims to reveal the mechanisms of hepatocyte apoptosis induced by dietary feeding. Eighteen midlactating goats were randomly divided into three groups: the low concentrate group (LC), the high concentrate group (HC), and the sodium butyrate (SB)-supplemented group (SHC). After 10 weeks, the HC diet successfully induced subacute ruminal acidosis (SARA), which increased the lipopolysaccharide (LPS) and cytokine concentrations and the expression of genes and proteins related to inflammation and apoptosis. The addition of SB to the HC diet notably decreased the levels of those parameters. Additionally, Bcl2 mRNA and protein expression was lower in the HC group than those in the LC and SHC groups. Furthermore, the HC diet reduced the percentages of caspase 3 and 8 promoter methylation compared to those of goats fed the LC diet, whereas the SHC diet partially recovered the methylation ratio to reduce caspase 3 and 8 expression. Collectively, HC diet-induced SARA caused hepatocyte apoptosis via activating the extrinsic apoptosis pathway, whereas dietary addition of SB depressed the inflammatory response and attenuated hepatocyte apoptosis. DNA methylation contributed to regulation of the expression of key apoptotic genes in the livers of lactating goats.