Characterization and localization of human renal kininogen.
Characterization and localization of human renal kininogen.
复制标题
人肾激肽原的表征和定位。
DOI：
10.1016/s0021-9258(19)68671-0
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
J. Pisano
中科院分区：
文献类型：
--
作者：
D. Proud;M. Perkins;J. Pierce;K. Yates;P. Highet;P. L. Herring;M. Mangkornkanok;R. Bahu;F. Carone;J. Pisano
以人血浆激肽原(HMW)和低分子激肽原(LMW)免疫同源的重(H)链为底物，用固定化抗体批量吸附从人尿中分离出激肽原。氯化胍洗脱液中的所有可释放激动素都与凝胶过滤组分中的激肽原抗原有关。洗脱液经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法可见主要染色条带和抗原带，与血浆LMW激肽原的主要形式相对应。此外，尿和血浆LMW激肽原的碱性圆盘凝胶电泳法得到的染色模式和抗原谱惊人地相似。用H链抗体进行间接免疫荧光染色，可在人肾远端小管、皮质集合管和髓质集合管的细胞内观察到胞浆染色。用抗血浆HMW激肽原轻链(L)的抗体不能观察到荧光，放射免疫法也没有发现完整的HMW激肽原。提示肾脏是尿激肽原的来源，而尿中的L链抗原可能是血浆高分子激肽原的滤过性降解产物。
Kininogen was isolated from human urine by batch adsorption with immobilized antibody to the immunologically identical heavy (H) chains of both high molecular weight (HMW) and low molecular weight (LMW) human plasma kininogens. All releasable kinin in the guanidinium chloride eluate was associated with kininogen antigen in gel filtration fractions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the eluate gave major stained and antigenic bands corresponding to the major form of plasma LMW kininogen. Also, the staining patterns and antigenic profiles obtained upon alkaline disc gel electrophoresis of the urinary and plasma LMW kininogens were strikingly similar. When antibody to H chain was used in an indirect immunofluorescence technique, cytoplasmic staining was observed in cells of distal tubules and c cortical and medullary collecting ducts of human kidneys. No fluorescence was observed using antibody to the unique light (L) chain of plasma HMW kininogen and no intact HMW kininogen was found in urine by radioimmunoassay. We conclude that the kidney is a source of urinary kininogen, while the L chain antigen in urine probably represents filtered degradation products of plasma HMW kininogen.