A recessive mutation in the RUB1-conjugating enzyme, RCE1, reveals a requirement for RUB modification for control of ethylene biosynthesis and proper induction of basic chitinase and PDF1.2 in Arabidopsis
A recessive mutation in the RUB1-conjugating enzyme, RCE1, reveals a requirement for RUB modification for control of ethylene biosynthesis and proper induction of basic chitinase and PDF1.2 in Arabidopsis
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DOI：
10.1111/j.1365-313x.2004.02068.x
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发表时间：
2004-05-01
期刊：
影响因子：
7.2
通讯作者：
Cancel, JD
中科院分区：
文献类型：
--
作者：
Larsen, PB;Cancel, JD
通过筛选黄化的拟南芥幼苗的突变体与异常乙烯相关的表型，我们确定了一个突变体，显示功能的乙烯介导的三重响应，即使在没有乙烯。进一步的表征表明，观察到的表型为黑暗生长的幼苗的突变体是可逆的，通过防止乙烯的感知，是依赖于适度增加乙烯生产与增加1-氨基环丙烷-1-羧酸（ACC）氧化酶（ACO）的活性在下胚轴。叶片的突变体的分子特征显示严重受损的诱导基本几丁质酶（chiB）和植物防御素（PDF）1.2茉莉酸和/或乙烯处理后。突变的定位克隆导致在RCE 1中鉴定出49-bp缺失（与泛素1（RUB 1）-缀合酶相关），其已被证明负责RUB 1与SCF（Skpl Cdc 53 F-box）泛素连接酶复合物的共价连接以改变其活性。我们的分析与rce 1 -2证明了一个以前未知的需求RUB 1修饰调节乙烯生物合成和适当的诱导拟南芥中的防御相关基因。
By screening etiolated Arabidopsis seedlings for mutants with aberrant ethylene-related phenotypes, we identified a mutant that displays features of the ethylene-mediated triple response even in the absence of ethylene. Further characterization showed that the phenotype observed for the dark-grown seedlings of this mutant is reversible by prevention of ethylene perception and is dependent on a modest increase in ethylene production correlated with an increase in 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO) activity in the hypocotyl. Molecular characterization of leaves of the mutant revealed severely impaired induction of basic chitinase (chiB) and plant defensin (PDF)1.2 following treatment with jasmonic acid and/or ethylene. Positional cloning of the mutation resulted in identification of a 49-bp deletion in RCE1 (related to ubiquitin 1 (RUB1)-conjugating enzyme), which has been demonstrated to be responsible for covalent attachment of RUB1 to the SCF (Skpl Cdc 53 F-box) ubiquitin ligase complex to modify its activity. Our analyses with rce1-2 demonstrate a previously unknown requirement for RUB1 modification for regulation of ethylene biosynthesis and proper induction of defense-related genes in Arabidopsis.