Assessment of midgut enteroendocrine peptide complement in the honey bee, Apis mellifera
Assessment of midgut enteroendocrine peptide complement in the honey bee, Apis mellifera
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DOI：
10.1016/j.ibmb.2019.103257
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发表时间：
2020-01-01
影响因子：
3.8
通讯作者：
Christie, Andrew E.
中科院分区：
文献类型：
--
作者：
Christie, Andrew E.
多肽调节所有动物的生理/行为控制系统。在节肢动物中，中肠上皮内分泌细胞是这些信号剂的最大来源之一。目前，对节肢动物中肠肠内分泌肽的组成机制尚不清楚。虽然许多技术可用于肽结构鉴定，但在硅转录组挖掘是一种已广泛用于节肢动物神经肽dome预测；该策略尚未用于节肢动物肠内分泌肽的大规模发现。在这里，一个组织特异性转录组被用来评估假定的肠内分泌肽补体在蜜蜂，蜜蜂，中肠。对42个肽家族成员的转录本进行了搜索，在该组合中发现了15个组的表达证据：脂肪动力激素、化抑素A、化抑素C、bursicon、CCHamide、CNMamide、利尿激素31、利尿激素44、胰岛素样肽、肌抑制素、神经肽F、色素分散激素、焦激肽、短神经肽F和速激肽相关肽。从中肠转录本中推断出的蛋白质序列与先前沉积在NCBI中的api前/前原激素的序列相同，或几乎相同，这增加了对报告数据准确性的信心。从推断的前体蛋白中预测了75个肽，其中26个是已知肽家族的成员。与先前发表的质谱数据的比较支持许多预测的api肽的存在。本研究首次利用转录组学方法预测了节肢动物中肠肽肽的功能，为研究具有重要生态/经济意义的Apis中肠的肠内分泌肽信号提供了强有力的新资源。
Peptides modulate physiological/behavioral control systems in all animals. In arthropods, midgut epithelial endocrine cells are one of the largest sources of these signaling agents. At present, little is known about the identity of the peptides that form arthropod midgut enteroendocrine peptidomes. While many techniques can be used for peptide structural identification, in silico transcriptome mining is one that has been used extensively for arthropod neuropeptidome prediction; this strategy has yet to be used for large-scale arthropod enteroendocrine peptide discovery. Here, a tissue-specific transcriptome was used to assess putative enteroendocrine peptide complement in the honey bee, Apis mellifera, midgut. Searches for transcripts encoding members of 42 peptide families were conducted, with evidence of expression for 15 groups found in the assembly: adipokinetic hormone, allatostatin A, allatostatin C, bursicon, CCHamide, CNMamide, diuretic hormone 31, diuretic hormone 44, insulin-like peptide, myosuppressin, neuropeptide F, pigment dispersing hormone, pyrokinin, short neuropeptide F, and tachykinin-related peptide. The proteins deduced from the midgut transcripts are identical in sequence, or nearly so, to those of Apis pre/preprohormones deposited previously into NCBI, providing increased confidence in the accuracy of the reported data. Seventy-five peptides were predicted from the deduced precursor proteins, 26 being members of known peptide families. Comparisons to previously published mass spectrometric data support the existence of many of the predicted Apis peptides. This study is the first prediction of an arthropod midgut peptidome using transcriptomics, and provides a powerful new resource for investigating enteroendocrine peptide signaling within/from the Apis midgut, a species of significant ecological/economic importance.