Regulation of embryonic endochondral ossification by Smurf2.
Regulation of embryonic endochondral ossification by Smurf2.
复制标题
Smurf2 对胚胎软骨内骨化的调节。
DOI：
10.1002/jor.20563
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Rosier,RandyN
中科院分区：
文献类型：
--
作者：
Wu,Qiuqian;Wang,Meina;Zuscik,MichaelJ;Chen,Di;O'Keefe,RegisJ;Rosier,RandyN
Smurf2 is an E3 ubiquitin ligase that targets TGF‐β receptor activated Smad2 and Smad3 for the proteasome in primary articular chondrocytes, thus stimulating their hypertrophic differentiation. Comparatively, how Smurf2 functions in growth plate chondrocytes in a developing long bone is an open question. In this study, we measured the mRNA levels of endogenousSmurf2and type X collagen in chick growth plate at different embryonic stages to monitor the correlation between the level of Smurf2 expression and chondrocyte maturational stage. We found that high levels of Smurf2 were associated with the differentiative and proliferative stages, while Smurf2 levels were thereafter decreased as the chondrocytes matured toward hypertrophy. In addition, we injectedSmurf2‐RCAS into chick wing buds at HH stage 20–23 and examined how the ectopic overexpression ofSmurf2in condensing chondrogenic mesenchyme affects the subsequent process of chondrocyte maturation and ossification during embryonic development. Histological analysis showed that overexpression of Smurf2 in a developing wing bud accelerated chondrocyte maturation and endochondral ossification, which may result from a decrease in TGF‐β signaling in the infected chondrocytes withSmurf2‐RCAS. © 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 26:704–712, 2008
