The pro-myogenic environment provided by whole organ scale acellular scaffolds from skeletal muscle
The pro-myogenic environment provided by whole organ scale acellular scaffolds from skeletal muscle
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DOI：
10.1016/j.biomaterials.2011.07.016
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发表时间：
2011-11-01
期刊：
影响因子：
14
通讯作者：
Coletti, Dario
中科院分区：
文献类型：
--
作者：
Perniconi, Barbara;Costa, Alessandra;Coletti, Dario
在寻求用于替换由创伤或病理状况引起的大的骨骼肌缺损的可移植构建体的过程中，已经进行了几次尝试以获得高度定向的、血管化的和功能性的骨骼肌。脱细胞支架是一种具有广阔应用前景的组织工程生物材料。然而，脱细胞骨骼肌细胞外基质（ECM）的生产，储存和宿主-供体相互作用方面的特点很差。我们已经从小鼠的各种骨骼肌中制备了全器官规模的脱细胞支架。脱细胞支架保留了原始组织的化学和结构特征，包括血管床。支架可以在组织培养级条件下在+4 ° C或+37 ° C下无菌储存数周。当移植到野生型小鼠体内时，移植物稳定了几周，同时被炎症细胞和干细胞定殖。我们证明，脱细胞支架本身代表了支持肌纤维从头形成的促生肌环境，可能来源于具有生肌潜能的宿主细胞。通过免疫抑制治疗增强植入物内的肌生成。我们的工作突出了这个利基在组织工程应用中的基础作用，并揭示了基于脱细胞组织的同种异体移植物在再生医学中的临床潜力。(C)2011爱思唯尔有限公司版权所有。
In the pursuit of a transplantable construct for the replacement of large skeletal muscle defects arising from traumatic or pathological conditions, several attempts have been made to obtain a highly oriented, vascularized and functional skeletal muscle. Acellular scaffolds derived from organ decellularization are promising, widely used biomaterials for tissue engineering. However, the acellular skeletal muscle extra cellular matrix (ECM) has been poorly characterized in terms of production, storage and host-donor interactions. We have produced acellular scaffolds at the whole organ scale from various skeletal muscles explanted from mice. The acellular scaffolds conserve chemical and architectural features of the tissue of origin, including the vascular bed. Scaffolds can be sterilely stored for weeks at +4 degrees C or +37 degrees C in tissue culture grade conditions. When transplanted in wt mice, the grafts are stable for several weeks, whilst being colonized by inflammatory and stem cells. We demonstrate that the acellular scaffold per se represents a pro-myogenic environment supporting de novo formation of muscle fibers, likely derived from host cells with myogenic potential. Myogenesis within the implant is enhanced by immunosuppressive treatment. Our work highlights the fundamental role of this niche in tissue engineering application and unveils the clinical potential of allografts based on decellularized tissue for regenerative medicine. (C) 2011 Elsevier Ltd. All rights reserved.