Liver-derived extracellular matrix as a biologic scaffold for acute vocal fold repair in a canine model.
Liver-derived extracellular matrix as a biologic scaffold for acute vocal fold repair in a canine model.
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肝源性细胞外基质作为犬模型急性声带修复的生物支架。
DOI：
10.1002/lary.20575
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Rosen,ClarkA
中科院分区：
文献类型：
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作者：
Gilbert,ThomasW;Agrawal,Vineet;Gilbert,MarkR;Povirk,KatherineM;Badylak,StephenF;Rosen,ClarkA
Objectives/HypothesisThe研究的目的是评估天然来源的肝细胞外基质（L-ECM）支架修复急性损伤的声带固有层在犬model.MethodsThe声带固有层被删除双侧在四只狗。每只犬中的一个声带用猪L-ECM支架修复，选择该支架是因为其含有肝细胞生长因子，一种有助于声带愈合的抗纤维化生长因子。另一个声带没有治疗。在手术后3个月，进行形态学和组织学分析，以评估声带的形状，胶原蛋白密度，胶原蛋白成分，弹性纤维含量，和glycosaminoglycan contents.ResultsThe L-ECM治疗的声带表现出增加的胶原蛋白密度在声带的浅表方面（P<0.05）。与未治疗组相比，L-ECM治疗的声带也显示胶原蛋白III/I比率增加（P< .05）。然而，弹性纤维含量被发现在两组中增加，和糖胺聚糖含量在两组中减少相比，正常声带（P<0.05），没有检测到组间差异conclusionsThe L-ECM支架没有恢复的生化组成或组织学外观损伤声带相比，正常。然而，胶原蛋白III/I和弹性纤维含量的比率增加表明，L-ECM导致结缔组织的形成，与未处理相比，结缔组织可能更柔韧。需要进行额外的调查，包括功能评估。喉镜，2009年
Objectives/HypothesisThe objective of the study was to evaluate a naturally derived liver extracellular matrix (L‐ECM) scaffold for repair of an acute injury to the vocal fold lamina propria in a canine model.MethodsThe vocal fold lamina propria was removed bilaterally in four dogs. One vocal fold in each dog was repaired with a porcine L‐ECM scaffold, which was chosen because it contains hepatocyte growth factor, an antifibrotic growth factor that aids the healing of vocal folds. The other vocal fold was left untreated. At 3 months after surgery, morphologic and histologic analysis was performed to assess the vocal fold shape, collagen density, collagen composition, elastic fiber content, and glycosaminoglycan content.ResultsThe L‐ECM–treated vocal fold showed increased collagen density in the superficial aspect of the vocal fold (P< .05). The L‐ECM–treated vocal fold also showed an increased collagen III/I ratio as compared to the nontreated group (P< .05). However, the elastic fiber content was found to be increased in both groups, and the glycosaminoglycan content was decreased in both groups as compared to the normal vocal fold (P< .05) with no differences detected between groups.ConclusionsThe L‐ECM scaffold did not restore the biochemical composition or histologic appearance of the injured vocal fold as compared to normal. However, the increased ratio of collagen III/I and elastic fiber content suggests that L‐ECM leads to formation of connective tissue that may be more pliable as compared to no treatment. Additional investigation, including functional assessment, is warranted. Laryngoscope, 2009