THE HUMAN MID-SIZE NEUROFILAMENT SUBUNIT - A REPEATED PROTEIN-SEQUENCE AND THE RELATIONSHIP OF ITS GENE TO THE INTERMEDIATE FILAMENT GENE FAMILY
THE HUMAN MID-SIZE NEUROFILAMENT SUBUNIT - A REPEATED PROTEIN-SEQUENCE AND THE RELATIONSHIP OF ITS GENE TO THE INTERMEDIATE FILAMENT GENE FAMILY
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DOI：
10.1002/j.1460-2075.1987.tb02409.x
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发表时间：
1987-06-01
期刊：
影响因子：
11.4
通讯作者：
NELSON, DL
中科院分区：
文献类型：
--
作者：
MYERS, MW;LAZZARINI, RA;NELSON, DL
我们报告的分离和测序的cDNA和基因组克隆的两个大亚基的人核丝，NF-M。对序列的分析使我们能够研究这种蛋白质的羧基末端尾部的结构，并将其与小神经丝以及其他中间丝进行比较。该蛋白的羧基末端区域含有连续重复六次的富含脯氨酸和丝氨酸的13个氨基酸序列。在每个重复单元内是序列Lys-Ser-Pro-Val的两个较小的重复。四个氨基酸重复可以代表已知高度磷酸化的蛋白质区域中的激酶识别位点。我们还注意到在蛋白质的羧基末端存在一个额外的七肽重复。这60个氨基酸的区域可能参与卷曲螺旋相互作用，类似于促进杆区域中的细丝形成的那些。人类基因只有两个内含子。它们的位置对应于在小鼠的小神经丝中发现的三个内含子中的两个。因此，哺乳动物的三个神经丝基因中的两个具有类似的结构，这些结构与其他中间丝的结构完全不同。这一发现表明神经丝亚基的共同起源，其与其他中间丝基因的进化关系尚不确定。
We report the isolation and sequence of cDNA and genomic clones for one of the two large subunits of human nuerofilament, NF-M. Analysis of the sequence has allowed us to investigate the structure of the carboxy-terminal tail of this protein, and to compare it to that of the small neurofilament as well as to other intermediate filaments. The carboxy-terminal region of the protein contains a 13 amino acid proline- and serine-rich sequence repeated six time in succession. Withn each repeat unit are two smaller repeats of the sequence Lys-Ser-Pro-Val. The four amino acid repeat may represent a kinase recognition site in a region of the protein that is known to be highly phosphorylated. We also note the presence of an additional heptad repeat at the extreme carboxy terminus of the protein. This region of 60 amino acids may be involved in coiled-coil interactions similar to those that facilitate the filament formation in the rod region. The human gene contains only two introns. Their positions correspond to two of the three introns found in the small neurofilament of the mouse. Thus, two of the three neurofilament genes of mammals have similar structures which are quite different from those of the other intermediate filaments. This finding suggests a common origin of the neurofilament subunits, whose evolutionary relationship to other intermediate filament genes is uncertain.