Induction of phosphodiesterase by cyclic adenosine 3':5'-monophosphate in differentiating Dictyostelium discoideum amoebae.
Induction of phosphodiesterase by cyclic adenosine 3':5'-monophosphate in differentiating Dictyostelium discoideum amoebae.
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环腺苷 3:5-单磷酸诱导磷酸二酯酶分化盘基网柄菌阿米巴。
DOI：
10.1016/s0021-9258(19)40919-8
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
C. Klein
中科院分区：
文献类型：
--
作者：
C. Klein
将环腺苷 3':5'-一磷酸添加到盘基网柄菌阿米巴变形虫的饥饿培养基中可诱导这些细胞的细胞内和细胞外磷酸二酯酶活性。诱导的酶活性比未用环核苷酸诱导的饥饿细胞中的酶活性早几个小时出现。在这两种情况下，放线菌酮、放线菌素 D 和道诺霉素都会抑制酶的出现。核苷酸诱导的和未诱导的对照细胞的胞外酶的 KmS 是相同的。酶活性的诱导似乎对环腺苷 3':5'-单磷酸具有特异性，因为环鸟苷 3':5'-单磷酸以及其他核苷酸没有效果。在环腺苷 3':5'-单磷酸诱导的细胞和对照细胞之间没有观察到 N-乙酰氨基葡萄糖苷酶的活性或排泄或细胞外磷酸二酯酶的抑制存在差异。可以排除环腺苷 3':5'-单磷酸对磷酸二酯酶的直接激活，因为向细胞裂解物中添加该核苷酸对酶活性没有影响。
Cyclic adenosine 3':5'-monophosphate added to the starvation media of Dictyostelium discoideum amoebae induces both intracellular and extracellular phosphodiesterase activities of these cells. The induced enzyme activity appears several hours earlier than that in starved cells which have not been induced with cyclic nucleotide. In both cases, the appearance of enzyme is inhibited by cycloheximide, and actinomycin D, and daunomycin. The KmS for the extracellular enzyme(s) of nucleotide-induced and uninduced control cells are identical. The induction of enzyme activity seems specific for cyclic adenosine 3':5'-monophosphate since cyclic guanosine 3':5'-monophosphate, as well as other nucleotides, have no effect. No differences in the activity or excretion of either N-acetylglucosaminidase or the inhibitory of the extracellular phosphodiesterase are observed between cyclic adenosine 3':5'-monophosphate-induced and control cells. A direct activation of phosphodiesterase by cyclic adenosine 3':5'-monophosphate can be excluded, since the addition of this nucleotide to cell lysates has no effect on the enzyme activity.