Clinical significance of miR-1180-3p in hepatocellular carcinoma: a study based on bioinformatics analysis and RT-qPCR validation
Clinical significance of miR-1180-3p in hepatocellular carcinoma: a study based on bioinformatics analysis and RT-qPCR validation
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miR-1180-3p在肝细胞癌中的临床意义：基于生物信息学分析和RT-qPCR验证的研究
DOI：
10.1038/s41598-020-68450-z
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发表时间：
2020-07
影响因子：
4.6
通讯作者：
Yu Hongping
中科院分区：
文献类型：
--
作者：
Zhou Zihan;Zhou Xianguo;Jiang Yanji;Qiu Moqin;Liang Xiumei;Lin Qiuling;Guo Qian;Nong Cunli;Huo Rongrui;Chen Qian;Liu Haizhou;Liu Yingchun;Zhu Shaoliang;Wang Mengyun;Yu Hongping
miRNAs在人类肿瘤发生中起着不可或缺的作用。在包括肝细胞癌（HCC）在内的几种类型的癌症中已经观察到失调的miR-1180- 3 p。本研究旨在探讨miR-1180- 3 p表达水平与HCC患者临床特征和总生存期的相关性。使用RT-qPCR方法验证从GEO和TCGA数据库中选择的miR-1180- 3 p的表达和临床意义。利用3个miRNA靶基因预测数据库获得miR-1180- 3 p的靶基因，并利用WebGestalt在线工具分析其功能。88例肝癌组织中miR-1180- 3 p的表达显著高于非肿瘤肝组织（0.004 ± 0.009 vs. 0.002 ± 0.002，t =-2.099，P = 0.038）。miR-1180- 3 p的表达水平与肿瘤数目（χ2= 9.157，P = 0.006）和MVI（χ2= 11.354，P = 0.003）显著相关。基于Kaplan-Meier分析，miR-1180高表达患者的总生存期短于miR-1180- 3 p低表达患者（P= 0.002）。多因素考克斯分析显示miR-1180- 3 p表达是影响总生存率的独立预后因素（HR = 13.36，95%CI 1.16，153.69，P = 0.038）。此外，从3个预测数据库中共找到733个miR-1180- 3 p靶基因。GO分析表明，靶基因与肿瘤的增殖和恶性程度密切相关。KEGG分析表明，靶基因在几个关键的癌症相关信号通路中富集，包括癌症中的通路、Ras信号通路和MAPK信号通路。总之，我们证明miR-1180- 3 p在HCC中上调，并且与不良预后相关。因此，miR-1180- 3 p可能作为HCC的预后标志物是有用的。
miRNAs play an indispensable role in human carcinogenesis. Dysregulated miR-1180-3p has been observed in several types of cancer, including hepatocellular carcinoma (HCC). This study intends to correlate the expression level of miR-1180-3p with clinical features and overall survival in HCC patients. The expression and clinical significance of miR-1180-3p, selected from GEO and TCGA databases, were verified using an RT-qPCR method. The target genes of miR-1180-3p were obtained using 3 miRNA target gene prediction databases, and their functions were analyzed using the online tool WebGestalt. miR-1180-3p expression was significantly upregulated in 88 HCC tissues compared with non-tumor liver tissues (0.004 ± 0.009 vs. 0.002 ± 0.002, t = − 2.099,P= 0.038). Additionally, we found that the expression levels of miR-1180-3p were significantly correlated with tumor number (χ2= 9.157,P= 0.006) and MVI (χ2= 11.354,P= 0.003). Based on Kaplan–Meier analysis, patients with high miR-1180 expression had a shorter overall survival than those with low miR-1180-3p expression (P= 0.002). Furthermore, multivariate Cox analyses indicated that miR-1180-3p expression was an independent prognostic factor for overall survival (HR = 13.36, 95% CI 1.16, 153.69,P= 0.038). In addition, a total of 733 target genes of miR-1180-3p were found from three prediction databases. The GO analyses demonstrated that the target genes were closely related to the proliferation and malignancy of tumors. The KEGG analysis showed that target genes were enriched in several key cancer-related signaling pathways, including the Pathways in cancer, the Ras signaling pathway, and the MAPK signaling pathway. In conclusion, we demonstrate that miR-1180-3p is upregulated in HCC and is associated with a poor prognosis. Thus, miR-1180-3p might be useful as a prognostic marker for HCC.
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影响因子：
14.9
作者：
Ritchie ME;Phipson B;Wu D;Hu Y;Law CW;Shi W;Smyth GK
通讯作者：
Smyth GK
影响因子：
3.7
作者：
Watahiki A;Wang Y;Morris J;Dennis K;O'Dwyer HM;Gleave M;Gout PW;Wang Y
通讯作者：
Wang Y
影响因子：
5.8
作者：
Wen DY;Lin P;Liang HW;Yang X;Li HY;He Y;Yang H;Chen G
通讯作者：
Chen G
影响因子：
3.4
作者：
Falzone, Luca;Romano, Giovanni L.;Candido, Saverio
通讯作者：
Candido, Saverio
DOI：
10.1007/978-1-4939-7435-1
发表时间：
2018
期刊：
--
影响因子：
--
作者：
Wei Wu
通讯作者：
Wei Wu