Proteomic changes occurring in the malaria mosquitoes Anopheles gambiae and Anopheles stephensi during aging
Proteomic changes occurring in the malaria mosquitoes Anopheles gambiae and Anopheles stephensi during aging
复制标题
DOI：
10.1016/j.jprot.2015.06.008
复制
发表时间：
2015-08-03
影响因子：
3.3
通讯作者：
Hugo, Leon E.
中科院分区：
文献类型：
--
作者：
Sikulu, Maggy T.;Monkman, James;Hugo, Leon E.
蚊子的年龄是其传播病原体的能力和对杀虫剂的抗性的关键决定因素。我们研究了在疟疾蚊子、冈比亚按蚊和斯氏按蚊的头部和胸部中发现的蛋白质丰度随年龄的变化。使用二维差异凝胶电泳评估蛋白质表达变化，并使用基质辅助激光解吸电离串联飞行时间质谱或与线性离子阱偶联的毛细管高压液相色谱法确定差异表达蛋白质的身份。（LTQ）-Orbitrap XL混合质谱仪。通过半定量Western blot分析，分别鉴定出19个和9个与年龄相关的蛋白质点。stephensi和A.冈比亚。在随年龄下调的蛋白质中，有ADF/Cofilin、细胞色素c1、热休克蛋白-70和真核细胞翻译起始因子5A（eIF 5a）的同源物。随着年龄的增长而上调的蛋白质可能包括甲基丙二酸半醛脱氢酶、电压依赖性阴离子选择性通道和果糖二磷酸醛缩酶。半定量Western印迹分析证实了通过2D DIGE观察到的eIF 5a和ADF/Cofilin的表达模式。建议进一步开展工作，以确定这些生物标志物是否对感染、吸血和杀虫剂抗性具有鲁棒性。生物学意义：在这项研究中，我们已经确定了几个蛋白质的特征变化丰富的冈比亚和A。在他们的衰老过程中。这些变化可能突出了蚊子年龄与影响疟原虫传播和蚊子控制的因素之间关系的潜在机制。这些物种和主要登革热媒介埃及伊蚊之间蛋白质丰度变化的相似性揭示了衰老特异性蛋白质调控的保守模式。(C)2015 Elsevier B. V.版权所有。
The age of mosquitoes is a crucial determinant of their ability to transmit pathogens and their resistance to insecticides. We investigated changes to the abundance of proteins found in heads and thoraces of the malaria mosquitoes Anopheles gambiae and Anopheles stephensi as they aged. Protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry or capillary high-pressure liquid chromatography coupled with a linear ion-trap (LTQ)-Orbitrap XL hybrid mass spectrometer. Protein biomarkers were validated by semi quantitative Western blot analysis.Nineteen and nine age dependent protein spots were identified for A. stephensi and A. gambiae, respectively. Among the proteins down-regulated with age were homologs of ADF/Cofilin, cytochome c1, heat shock protein-70 and eukalyotic translation initiation factor 5A (eIF5a). Proteins up-regulated with age included probable methylmalonate-semialdehyde dehydrogenase, voltage-dependent anion-selective channel and fructose bisphosphate aldolase. Semi quantitative Western blot analysis confirmed expression patterns observed by 2D DIGE for eIF5a and ADF/Cofilin. Further work is recommended to determine whether these biomarkers are robust to infection, blood feeding and insecticide resistance. Robust biomarkers could then be incorporated into rapid diagnostic assays for ecological and epidemiological studies.Biological significance: In this study, we have identified several proteins with characteristic changes in abundance in both A gambiae and A. stephensi during their aging process. These changes may highlight underlying mechanisms beneath the relationship between mosquito age and factors affecting Plasmodium transmission and mosquito control. The similarity of changes in protein abundance between these species and the primary dengue vector Aedes aegypti, has revealed conserved patterns of aging-specific protein regulation. (C) 2015 Elsevier B.V. All rights reserved.