Nucleotide sequence of bacteriophage T4 gene 23 and the amino acid sequence of its product.
Nucleotide sequence of bacteriophage T4 gene 23 and the amino acid sequence of its product.
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DOI：
10.1016/0022-2836(84)90019-6
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发表时间：
1984-12
影响因子：
5.6
通讯作者：
M. L. Parker;A. Christensen;A. Boosman;J. Stockard;E. Young;A. Doermann
中科院分区：
文献类型：
--
作者：
M. L. Parker;A. Christensen;A. Boosman;J. Stockard;E. Young;A. Doermann
我们用Maxam和吉尔伯特的方法和桑格的方法测定了噬菌体T4的基因23的核苷酸序列。另外，通过Edman降解完整蛋白质及其肽，确定了由基因23编码的主要衣壳蛋白的约80%的氨基酸残基的同一性。已在该序列中定位了15个基因23的琥珀突变位点，并确定了基因21、22和23的3′转录终止位点。
We have determined the nucleotide sequence of gene 23 of bacteriophage T4 by the methods of Maxam and Gilbert and of Sanger. The identities of approximately 80% of the amino acid residues of the major capsid protein which is encoded by gene 23 were determined additionally by Edman degradation of the intact protein and its peptides. Fifteen gene 23 amber mutation sites have been located within the sequence, and the 3′ transcription termination site for genes 21, 22 and 23 has been identified.