Cloning, expression and partial characterization of a gene encoding the S15a ribosomal protein of Taenia solium
Cloning, expression and partial characterization of a gene encoding the S15a ribosomal protein of Taenia solium
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DOI：
10.1007/s00436-003-1021-4
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发表时间：
2004-03-01
影响因子：
2
通讯作者：
Landa, A
中科院分区：
文献类型：
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作者：
Jiménez, L;Becerra, A;Landa, A
核糖体、核糖体蛋白（r-蛋白）、信使和转移RNA催化生物体中蛋白质的合成。为了了解和确定参与这一事件的猪带绦虫的成分，我们分离并鉴定了一个T。solium cDNA编码碱性核糖体蛋白S15 a（TsS 15 a）。TsS 15 a cDNA产生相对分子质量为14，988的蛋白质，其含有22.3%的碱性氨基酸。TsS 15 a蛋白与其他S15 a r蛋白的比较分析表明，该蛋白是高度保守的。在细菌中产生具有类似M-r的重组TsS 15 a蛋白。抗重组TsS 15 a的抗体与来自T.和所有测试的蠕虫。杂交研究表明存在两个基因编码的mRNA的0.5 kb。此外，该基因存在一个30 bp的内含子。我们的系统发育分析使用S15 a R-蛋白复制的拓扑结构报告的16/18 S rRNA。
Ribosomes, ribosomal proteins (r-proteins), and messenger and transfer RNAs catalyze the synthesis of proteins in organisms. To understand and define the components involved in this event in Taenia solium, we isolated and characterized a T. solium cDNA encoding the basic ribosomal protein S15a (TsS15a). The TsS15a cDNA produces a protein with M-r (relative molecular mass) 14,988, which contains 22.3% of basic amino acids. Analysis comparing TsS15a protein with other S15a r-proteins indicates that this protein is highly conserved. A recombinant TsS15a protein with similar M-r was produced in bacteria. Antibodies against recombinant TsS15a react with a 15-kDa protein in extracts from all life stages of T. solium and from all helminths tested. Hybridization studies showed the presence of two genes encoding a mRNA of 0.5 kb. Moreover, the gene presents an intron of 30 bp. Our phylogenetic analysis using S15a r-proteins reproduced the topologies reported for 16/18S rRNA.