An evaluation by sequential extraction of the proportions of collagen types from medium sized arteries.
An evaluation by sequential extraction of the proportions of collagen types from medium sized arteries.
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通过从中等大小的动脉中顺序提取胶原类型的比例进行评估。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
J. Borel
中科院分区：
文献类型：
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作者：
A. Szymanowicz;G. Bellon;G. Laurain;T. Delvincourt;A. Randoux;T. Caulet;J. Borel
从没有动脉粥样硬化证据的患者尸检中获得的人类髂总动脉样本，使用交替超声处理、胃蛋白酶消化和二硫苏糖醇还原，然后对提取物进行十二烷基硫酸钠聚丙烯酰胺凝胶电泳，分析其在各种类型胶原中的含量。总胶原的最终平均比例为66%被溶解。从电泳运行的密度扫描推断的不同胶原蛋白的比例的统计计算表明，当提取更完全时，提取了更大量的I型胶原蛋白。I型胶原是这些动脉中最难溶解的胶原，其最终比例可能高于总胶原的60%。
Samples of human Common Iliac Arteries, obtained post-mortem from patients without evidence of atherosclerosis, were analyzed for their content in the various types of collagens using alternatively sonication, pepsin digestion and dithiothreitol reduction followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis of the extracts. A final average proportion of 66% of the total collagen was solubilized. The statistical calculation of the proportions of the different collagens, deduced from the densitometric scannings of the electrophoretic runs, showed that a larger amount of type I was extracted when the extraction was more complete. Type I collagen appeared as the most insoluble collagen in these arteries and its final proportion was probably higher than 60% of the total collagen.