Distribution and Frequency of kdr Mutations within Anopheles gambiae s.I. Populations and First Report of the Ace. 1G119S Mutation in Anopheles arabiensis from Burkina Faso (West Africa)
Distribution and Frequency of kdr Mutations within Anopheles gambiae s.I. Populations and First Report of the Ace. 1G119S Mutation in Anopheles arabiensis from Burkina Faso (West Africa)
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DOI：
10.1371/journal.pone.0101484
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发表时间：
2014-07-31
期刊：
影响因子：
3.7
通讯作者：
Combary, Patrice
中科院分区：
文献类型：
--
作者：
Dabire, Roch K.;Namountougou, Moussa;Combary, Patrice
在布基纳法索（西非）三个生态气候区的 15 个地点进行了昆虫学调查，以评估赋予按蚊杀虫剂抗性的突变的当前分布和频率。冈比亚该国的人口。两种敲低 (kdr) 抗性突变变体（L1014F 和 L1014S）均与 ace-1 G119S 突变一起被鉴定，这些突变变体赋予拟除虫菊酯杀虫剂抗性，证实了 An.An. 中存在多种抗性机制。布基纳法索的冈比亚复合体。与上次调查相比，所有物种中 L1014F kdr 突变的频率似乎基本保持稳定且相对较高。相比之下，L1014S突变的分布和频率在An中显着增加。冈比亚全国大部分地区。此外，我们首次报告了 An.1 G116S 突变的鉴定。在 8 个地点收集的阿拉伯种群。这种突变赋予了对有机磷和氨基甲酸酯杀虫剂的抗性，此前仅在按蚊中报道过。冈比亚 S 和 M 分子形式。这一发现具有重要意义，因为有机磷酸酯和氨基甲酸酯被用于室内滞留喷雾剂（IRS）来控制疟疾媒介，作为使用拟除虫菊酯浸渍蚊帐的补充策略。两个 An 中都发生了三个靶位点抗性突变。冈比亚分子形式和现在的安。阿拉伯疟原虫对布基纳法索疟疾病媒种群的控制以及基于不同作用模式杀虫剂轮换的耐药性管理策略具有重大影响。
An entomological survey was carried out at 15 sites dispersed throughout the three eco-climatic regions of Burkina Faso (West Africa) in order to assess the current distribution and frequency of mutations that confer resistance to insecticides in An. gambiae s.I. populations in the country. Both knockdown (kdr) resistance mutation variants (L1014F and L1014S), that confer resistance to pyrethroid insecticides, were identified concomitant with the ace-1 G119S mutation confirming the presence of multiple resistance mechanisms in the An. gambiae complex in Burkina Faso. Compared to the last survey, the frequency of the L1014F kdr mutation appears to have remained largely stable and relatively high in all species. In contrast, the distribution and frequency of the L1014S mutation has increased significantly in An. gambiae s.I. across much of the country. Furthermore we report, for the first time, the identification of the ace.1 G116S mutation in An. arabiensis populations collected at 8 sites. This mutation, which confers resistance to organophosphate and carbamate insecticides, has been reported previously only in the An. gambiae S and M molecular forms. This finding is significant as organophosphates and carbamates are used in indoor residual sprays (IRS) to control malaria vectors as complementary strategies to the use of pyrethroid impregnated bednets. The occurrence of the three target-site resistance mutations in both An. gambiae molecular forms and now An. arabiensis has significant implications for the control of malaria vector populations in Burkina Faso and for resistance management strategies based on the rotation of insecticides with different modes of action.