Culturing Adult Neural Stem Cells: Application to the Study of Neurodegenerative and Neuropsychiatric Pathology
Culturing Adult Neural Stem Cells: Application to the Study of Neurodegenerative and Neuropsychiatric Pathology
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DOI：
10.1007/978-4-431-53945-2_10
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
van der Kooy, Derek
中科院分区：
文献类型：
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作者：
Hitoshi, Seiji;Kippin, Tod;van der Kooy, Derek
干细胞存在于许多，也许是所有的成体组织中，具有前体储存库的共同功能，可以介导细胞更新或修复损伤。在组织特异性干细胞群体中，神经干细胞特别适合研究，因为它们很容易在简单的培养系统中分离和培养，从而证明细胞自主自我更新和多能性。在这里，我们描述了最广泛使用的培养系统，神经球试验，用于揭示神经干细胞的这些基本特性，这些特性对特定的生长因子有反应，并在无血清培养基中增殖，形成克隆球集落。尽管存在一些缺陷和局限性，但在适当的条件下使用神经球分析可以提供有关神经干细胞动力学和行为的详细信息。此外，神经球测定和使用核苷酸类似物标记的组织化学分析可以用于估计生理和病理条件下成人大脑中神经干细胞的数量和细胞动力学。
Stem cells exist in many, perhaps all, adult tissues and serve the common function of a reservoir of precursors that can mediate cell turnover or repair damage. Among tissue-specific stem cell populations, neural stem cells are particularly amenable to study because they are readily isolated and cultured in simple culture systems that allow demonstration of cell-autonomous self-renewal and multipotentiality. Here, we describe the most widely employed culture system, the neurosphere assay, used to reveal these cardinal properties of neural stem cells which respond to specific growth factors and proliferate in serum-free media to form clonal sphere colonies. Although several pitfalls and limitations exist, the neurosphere assay can provide detailed information about kinetics and behavior of neural stem cells when utilized under appropriate conditions. Further, the neurosphere assay together with histochemical analysis using nucleotide analog labeling may be applied to estimate the number and cellular kinetics of neural stem cells in the adult brain under physiological and pathological conditions.