Incidence of fecal carriage of Listeria monocytogenes in three healthy volunteers:: A one-year prospective stool survey
Incidence of fecal carriage of Listeria monocytogenes in three healthy volunteers:: A one-year prospective stool survey
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DOI：
10.1007/s10096-002-0835-9
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发表时间：
2003-01-01
影响因子：
4.5
通讯作者：
Allerberger, F
中科院分区：
文献类型：
--
作者：
Grif, K;Patscheider, G;Allerberger, F
本研究的目的是确定健康成人粪便携带单核细胞增生李斯特菌的发病率。在2000年10月1日至2001年9月30日期间，从3名健康志愿者（1名男性和2名女性;年龄44岁、39岁和60岁）中共采集了868份粪便标本。使用Fraser肉汤和Palcam选择性琼脂平板进行培养。使用Probelia Listeria monocytogenes（BioRad，France）进行聚合酶链反应（PCR）。总体而言，李斯特菌检测到31 868（3.57%）粪便标本，使用PCR。31例阳性结果中有16例为单一事件，即，同一名病人在测试结果呈阳性的前一天及后一天所抽取的样本均呈阴性。连续两天阳性4次，连续三天阳性1次，连续四天阳性1次。868份粪便标本中10份（1.15%）培养出单核细胞增生李斯特菌。这些培养阳性样本，PCR检测均为阳性，共发生5次独立事件。使用自动核糖分型高达40个单菌落每个粪便标本，9/10的培养阳性样品产生一个以上的菌株。阳性结果无明显的季节聚集性。没有记录的单核细胞增生李斯特菌携带事件，所有这些都涉及血清型1/2a和1/2b，与显性疾病相吻合。PCR的结果表明，每人每年暴露于单核细胞增生李斯特菌的发生率为5至9次。平均而言，在健康成人中，经培养证实的粪便携带的发生率为每人每年两次单核细胞增生李斯特菌携带。粪便脱落持续时间较短（最长4天），这与奶制品工人常规粪便筛查作为预防奶牛场绦虫病的工具的适当性相矛盾。
The aim of this study was to establish the incidence of fecal carriage of Listeria monocytogenes in healthy adults. A total of 868 stool specimens from three healthy volunteers (1 male and 2 females; ages 44, 39, and 60 years) were collected between 1 October 2000 and 30 September 2001. Culture was performed using Fraser broth and Palcam selective agar plates. Polymerase chain reaction (PCR) was performed using Probelia Listeria monocytogenes (BioRad, France). Overall, Listeria monocytogenes was detected in 31 of the 868 (3.57%) stool specimens using PCR. Sixteen of the 31 positive results were single events, i.e., samples collected from the same patient the day before and the day after the positive result were both negative. Positive results on two consecutive days were found four times, on three consecutive days one time, and on four consecutive days one time. Listeria monocytogenes was cultured from 10 of 868 (1.15%) stool specimens. These culture-positive samples, all positive by PCR as well, accounted for five independent episodes. Using automated ribotyping on up to 40 single colonies per stool specimen, 9 of 10 culture-positive samples yielded more than one strain. There was no obvious seasonal clustering of positive results. None of the documented episodes of Listeria monocytogenes carriage, all of which involved serotypes 1/2a and 1/2b, coincided with overt illness. The results of PCR indicate an incidence of five to nine exposures to Listeria monocytogenes per person per year. On average, the incidence of culture-confirmed fecal carriage in healthy adults is two episodes of Listeria monocytogenes carriage per person per year. Fecal shedding was of short duration (maximum 4 days), which argues against the appropriateness of routine stool screening in dairy workers as a tool for prevention of listeriosis.