The diversity and expansion of the trans-sialidase gene family is a common feature in Trypanosoma cruzi clade members
The diversity and expansion of the trans-sialidase gene family is a common feature in Trypanosoma cruzi clade members
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DOI：
10.1016/j.meegid.2015.11.024
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发表时间：
2016-01-01
影响因子：
3.2
通讯作者：
da Silveira, Jose Franco
中科院分区：
文献类型：
--
作者：
Angel Chiurillo, Miguel;Cortez, Danielle R.;da Silveira, Jose Franco
转唾液酸酶（TS）是原生动物锥虫属（Trypanosoma）成员中描述的多态性蛋白超家族。在最近描述的8个TS组中，TS组I蛋白（其中一些具有催化活性）存在于亲缘关系较远的布氏锥虫和克氏锥虫系统发育分支中，而其他TS组仅在属于T.克鲁济分支。本研究分析了TS基因的库、分布及在不同种间的系统发育关系。cruzi进化枝基于序列相似性、多序列比对和树重建方法，使用借助基于PCR的策略获得或从基因组数据库检索的TS序列。我们包括以下代表性的T. cruzi进化枝：T. cruzi，T. Cruzi Tcbat、Cruzi Marinkellei锥虫、Dionisii锥虫、Rangeli锥虫和Conorhini锥虫。克隆的序列编码保守的TS蛋白基序Asp-box和VTIPNVxLYNR，但缺乏FRIP基序（在TS组I中保守）。霸王Conorhini序列的差异最大。IS探针与染色体条带的杂交模式证实了这些序列在T.克鲁济分支。分歧和关系分析将大多数TS序列置于T.克鲁兹进一步研究了TS组II的成员，其中包括T。cruzi表面糖蛋白参与宿主细胞的粘附和侵袭，显示T. cruzi Tcbat与T. cruzi基因型TcI。我们的分析表明，T. cruzi进化枝具有不同的脊椎动物宿主、载体和致病性，共享TS基因家族的广泛扩展和序列多样性。总之，我们的研究结果是一致的进化历史的T。cruzi进化枝和代表的分子进化和TS蛋白在锥虫中的作用的理解的贡献。(C)2015 Elsevier B. V.版权所有。
Trans-sialidase (TS) is a polymorphic protein superfamily described in members of the protozoan genus Trypanosoma. Of the eight TS groups recently described, TS group I proteins (some of which have catalytic activity) are present in the distantly related Trypanosoma brucei and Trypanosoma cruzi phylogenetic clades, whereas other TS groups have only been described in some species belonging to the T. cruzi clade. In the present study we analyzed the repertoire, distribution and phylogenetic relationships of TS genes among species of the T. cruzi clade based on sequence similarity, multiple sequence alignment and tree-reconstruction approaches using TS sequences obtained with the aid of PCR-based strategies or retrieved from genome databases. We included the following representative isolates of the T. cruzi clade from South America: T. cruzi, T. cruzi Tcbat, Trypanosoma cruzi marinkellei, Trypanosoma dionisii, Trypanosoma rangeli and Trypanosoma conorhini. The cloned sequences encoded conserved TS protein motifs Asp-box and VTVxNVxLYNR but lacked the FRIP motif (conserved in TS group I). The T. conorhini sequences were the most divergent The hybridization patterns of IS probes with chromosomal bands confirmed the abundance of these sequences in species in the T. cruzi clade. Divergence and relationship analysis placed most of the TS sequences in the groups defined in T. cruzi. Further examination of members of TS group II, which includes T. cruzi surface glycoproteins implicated in host cell attachment and invasion, showed that sequences of T. cruzi Tcbat grouped with those of T. cruzi genotype TcI. Our analysis indicates that different members of the T. cruzi clade, with different vertebrate hosts, vectors and pathogenicity, share the extensive expansion and sequence diversification of the TS gene family. Altogether, our results are congruent with the evolutionary history of the T. cruzi clade and represent a contribution to the understanding of the molecular evolution and role of TS proteins in trypanosomes. (C) 2015 Elsevier B.V. All rights reserved.