An Optimized Zebrafish Nursery Feeding Regimen Improves Growth Rates and Labor Costs.
An Optimized Zebrafish Nursery Feeding Regimen Improves Growth Rates and Labor Costs.
复制标题
优化的斑马鱼保育饲养方案可提高生长速度并降低劳动力成本。
DOI：
10.1089/zeb.2021.0030
复制
发表时间：
2021
期刊：
影响因子：
2
通讯作者：
Blaser,BradleyW
中科院分区：
文献类型：
--
作者：
Collins,ToiA;Cabrera,Shelby;Teets,Emily;Shaffer,Jami;Blaser,BradleyW
为斑马鱼 (Danio rerio) 幼虫设定营养标准，以最大限度地提高生长、生存和繁殖成功率是一项挑战。我们通过比较 Gemma Micro 75 颗粒饲料和活体 L 型轮虫（褶皱臂尾轮虫）在受精后 9 天 (dpf) 开始的 3 种喂养方案中评估不同喂养方案对斑马鱼幼虫的影响：活体饲料的推注饲喂 (BL)、活体饲料的连续饲喂 (CL) 和颗粒饲料 (PD)。受精后 4-5 周，PD 和 CL 组的动物比 BL 组长。 PD 组的身体深度也比活食组更大。各组之间的体重没有显着差异。繁殖力或性别比例也没有显着差异，这表明所有饲养方法都成功促进了有用鱼类繁殖种群的生长。此外，我们量化了与这些方法相关的设备、消耗品和劳动力成本，发现 PD 方案优于两种活食方案。这些数据表明，为斑马鱼幼鱼和幼鱼提供高营养密度的颗粒饲料是促进早期生长并降低与保育相关的成本和劳动力的有效手段。
Setting nutritional standards for larval zebrafish (Danio rerio) that maximize growth, survival, and reproductive success is challenging. We evaluated the effects of different feeding regimens on larval zebrafish by comparing Gemma Micro 75 pelleted diet and live-type L rotifers (Brachionus plicatilis) in 3 feeding regimens starting at 9 days postfertilization (dpf): bolus feeding of live diet (BL), continuous feeding of live diet (CL), and pelleted diet (PD). Animals in the PD and CL groups were longer than the BL group at 4–5 weeks postfertilization. The PD group was also greater in body depth than both live diet groups. There was no significant difference in weight between the groups. There were also no significant differences in fecundity or sex ratios indicating that all feeding methods successfully promote growth of a useful breeding stock of fish. In addition, we quantified the equipment, consumable, and labor costs associated with these methods, and found that the PD regimen was superior to both live diet regimens. These data suggest that providing a high nutrient-density pelleted diet to larval and juvenile zebrafish is an effective means to increase early growth and to decrease cost and labor associated with nursery care.