Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys.
Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys.
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DOI：
10.1038/sdata.2017.67
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发表时间：
2017
期刊：
影响因子：
9.8
通讯作者：
Saitou M
中科院分区：
文献类型：
--
作者：
Nakamura T;Yabuta Y;Okamoto I;Sasaki K;Iwatani C;Tsuchiya H;Saitou M
在哺乳动物中，多能性的发育和原始生殖细胞（PGCs）的特化主要使用小鼠作为模式生物进行研究。然而，哺乳动物物种之间的分歧，这样的过程已经开始认识到。人类和小鼠的胚胎着床前发育似乎相对相似，但着床后发育的方式和形态却有显著差异。然而，由于在相关发育阶段分析胚胎的困难，在灵长类动物中植入后的胚胎发生，包括PGCs的特化，一直未被探索。在这里，我们提出了一个全面的单细胞转录组数据集植入前和早期后胚胎细胞，PGCs和胚胎干细胞（ESC）的食蟹猴作为一个模型的高级灵长类动物。每个转录组的身份也通过其他方法如免疫荧光分析进行了严格验证。这里报告的信息将作为我们理解包括人类在内的灵长类动物发育生物学过程的基础。
In mammals, the development of pluripotency and specification of primordial germ cells (PGCs) have been studied predominantly using mice as a model organism. However, divergences among mammalian species for such processes have begun to be recognized. Between humans and mice, pre-implantation development appears relatively similar, but the manner and morphology of post-implantation development are significantly different. Nevertheless, the embryogenesis just after implantation in primates, including the specification of PGCs, has been unexplored due to the difficulties in analyzing the embryos at relevant developmental stages. Here, we present a comprehensive single-cell transcriptome dataset of pre- and early post-implantation embryo cells, PGCs and embryonic stem cells (ESCs) of cynomolgus monkeys as a model of higher primates. The identities of each transcriptome were also validated rigorously by other way such as immunofluorescent analysis. The information reported here will serve as a foundation for our understanding of a wide range of processes in the developmental biology of primates, including humans.