Growth hormone regulates expression of rat liver cytochrome P-450 15 beta at a pretranslational level.
Growth hormone regulates expression of rat liver cytochrome P-450 15 beta at a pretranslational level.
复制标题
生长激素在翻译前水平调节大鼠肝细胞色素 P-450 15 beta 的表达。
DOI：
10.1016/0006-291x(87)91422-7
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发表时间：
1987
影响因子：
3.1
通讯作者：
J. Gustafsson
中科院分区：
文献类型：
--
作者：
C. Macgeoch;E. Morgan;B. Cordell;J. Gustafsson
使用抗大鼠肝微粒体细胞色素 P-45015β-羟化酶的多克隆抗体筛选雌性大鼠肝脏 cDNA 表达文库。从阳性菌落中分离出的重组质粒 C-6 含有 1000 个碱基对的 cDNA，在 Northern 印迹分析中与单个 1.7 kb 类 mRNA 杂交。半定量狭缝印迹显示，雄性大鼠肝脏中该 mRNA 的水平比雌性大鼠低 17 倍。给雄性大鼠连续输注人类生长激素导致它们表达雌性水平的C-6 mRNA，而间歇性给予相同日剂量的激素则没有效果。发现 C-6 mRNA 水平与 15β-羟化酶蛋白水平和催化活性高度相关，表明 15β-羟化酶的性别分化、生长激素依赖性表达在翻译前的步骤受到调节。
Polyclonal antibodies to rat liver microsomal cytochrome P-45015β-hydroxylase were used to screen a female rat liver cDNA expression library. Recombinant plasmid C-6, isolated from a positive colony, contained a cDNA of 1000 base pairs which hybridized to a single, 1.7 kilobase class of mRNA on Northern blot analysis. Semi-quantitative slot blots showed that the level of this mRNA was 17-fold lower in male rat liver than in females. Continuous infusion of human growth hormone to male rats caused them to express female levels of C-6 mRNA, while the same daily dose of hormone given intermittently had no effect. A high degree of correlation of the levels of C-6 mRNA with 15β-hydroxylase protein levels and catalytic activities was found, demonstrating that the sexually differentiated, growth hormone-dependent expression of the 15β-hydroxylase is regulated at a step preceding translation.