CHARACTERIZATION AND LOCALIZATION OF THE EVEN-SKIPPED PROTEIN OF DROSOPHILA
CHARACTERIZATION AND LOCALIZATION OF THE EVEN-SKIPPED PROTEIN OF DROSOPHILA
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DOI：
10.1002/j.1460-2075.1987.tb04817.x
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发表时间：
1987-03-01
期刊：
影响因子：
11.4
通讯作者：
LEVINE, M
中科院分区：
文献类型：
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作者：
FRASCH, M;HOEY, T;LEVINE, M
在同源盒交叉同源性的基础上，我们分离出了果蝇成对规则基因even-skipped（eve）。Eve转录单位长度似乎小于1.5kb，并编码单个mRNA。1.4 KB.基因组和cDNA克隆的核苷酸序列表明，eve蛋白由376个氨基酸残基组成，并且其同源结构域仅共享.与先前表征的基因的同源结构域具有50%的氨基酸同一性。使用针对β-半乳糖苷酶融合蛋白我们发现Eve蛋白在细胞胚盘阶段以一系列七条横向条纹分布，并且主要定位于表达该基因的那些胚胎细胞的核区域内。原肠胚形成后，出现7个每周染色的eve表达条纹，导致由总共14个均匀间隔的条纹组成的短暂模式。在胚带伸长过程中，原有条纹和新条纹均逐渐消失。第二种表达模式出现在神经发生过程中，在每个腹神经节的神经元的离散子集中检测到eve蛋白。
On the basis of homeo box cross-homology we have isolated the pair-rule gene even-skipped (eve) of Drosophila. The eve transcription unit appears to be less than 1.5 kb in length, and encodes a single mRNA of .apprx. 1.4 kb. The nucleotide sequence of genomic and cDNA clones indicates that the eve protein is composed of 376 amino acid residues, and that its homeo domain shares only .apprx. 50% amino acid identity with the homeo domains of previously characterized genes. Using antibodies raised against a .beta.-galactosidase fusion protein we show that the eve protein is distributed in a series of seven transverse stripes at the cellular blastoderm stage, and is localized primarily within the nuclear regions of those embryonic cells that express the gene. After gastrulation, seven weekly stained stripes of eve expression appear, resulting in a transient pattern that consists of a total of 14 evenly spaced stripes. Both the original and new stripes gradually disappear during germ band elongation. A second expression pattern emerges during neurogenesis, whereby eve protein is detected in discrete subsets of neurons in each of the ventral ganglia.