Isolation of dammarane saponins from Panax notoginseng by high-speed counter-current chromatography
Isolation of dammarane saponins from Panax notoginseng by high-speed counter-current chromatography
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DOI：
10.1016/s0021-9673(03)00988-9
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发表时间：
2003-08-08
影响因子：
4.1
通讯作者：
Winterhalter, P
中科院分区：
文献类型：
--
作者：
Du, QZ;Jerz, G;Winterhalter, P
传统上从三七（五加科）根的粗提取物制备的中药制剂三七总带片含有高极性达玛烷皂苷，其使用高速逆流色谱（HSCCC）在制备规模下分离。在每次操作中，分离出5片片剂的283 mg甲醇提取物，分别得到157、17、13和56 mg纯的三七皂苷-Rb-1、三七皂苷-R-1、三七皂苷-Re和三七皂苷-Rg（1）。正己烷-正丁醇-水（3：4：7，v/v/v）用于HSCCC分离的两相溶剂系统。通过电喷雾质谱和核磁共振分析，确定了3个皂苷和1个三七皂苷的化学结构。(C)2003 Elsevier B. V.保留所有权利。
The Chinese phytomedicinal formulation Sanqi Zongdai Pian, traditionally prepared from crude extracts from roots of Panax notoginseng (Araliaceae), contains highly polar dammarane saponins which were separated at a preparative scale using high-speed counter-current chromatography (HSCCC). In each operation, 283 mg methanolic extract of five tablets was separated and yielded pure 157, 17, 13 and 56 mg of ginsenoside-Rb-1, notoginsenoside-R-1, ginsenoside-Re and ainsenoside-Rg(1), respectively, n-hexane-n-butanol-water (3:4:7, v/v/v) was used for the two-phase solvent system of the HSCCC separation. The chemical structures of three ginsenosides and one notoginsenoside were elaborated by means of electrospray ionization MS-MS and NMR analysis. (C) 2003 Elsevier B.V. All rights reserved.