Preparation of multivalent glycan micro- and nano-arrays: general discussion.
Preparation of multivalent glycan micro- and nano-arrays: general discussion.
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多价聚糖微阵列和纳米阵列的制备：一般讨论。
DOI：
10.1039/c9fd90062d
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发表时间：
2019
影响因子：
3.4
通讯作者：
Braunschweig A
中科院分区：
文献类型：
--
作者：
Braunschweig A
Adam Braunschweig开启了Jeffrey Gildersleve的论文讨论：在您对这篇讨论的贡献中，您已经表明蛋白质和多糖之间的亲和力敏感地依赖于糖-多糖间距、连接子组成、连接子长度等。因此，不同的微阵列对特定的城市和对特定凝集素的亲和力产生不同的结果。考虑到这种内在的复杂性，贪婪有没有“真正的价值”，可以最准确地反映这种识别是如何在生物学中发生的？杰夫·吉尔德斯利夫回答道：这是一个有趣的考虑。在生物学中，糖链决定簇可以存在于不同的环境中。亲和力将取决于行列式的结构和陈述。例如，一个凝集素可能有四个结合位点。在某些情况下，凝集素可能只能与两个结合位点结合。在其他设置中，凝集素可能使用其所有四个结合链来结合多聚糖。这两者在生物学上可能是相关的，但嗜好程度可能有很大的不同。因此，每个糖链决定簇的亲和力可能有许多“真值”。当将在糖链微阵列上测得的表观Kd值连接到生物系统时，我认为这是潜在的--“在适当的情况下，这种凝集素可以与表观Kd值X结合到这个糖链上”。它具有结合的潜力，但它将取决于其他因素，如载体链的性质以及多糖的间距和取向。
Adam Braunschweig opened the discussion of the paper by Jeffrey Gildersleeve: In your contribution to this Discussion, you have shown that the avidity between protein and glycan is sensitively dependent upon glycan–glycan spacing, linker composition, linker length, etc. As a result, different microarrays produce different results for speci city and avidity towards a particular lectin. Given this inherent complexity, is there a “true value” for avidity that re ects most accurately how this recognition may be occurring in biology?Jeff Gildersleeve answered: This is an interesting consideration. A glycan determinant can be present in varying contexts in biology. The avidity will depend on the structure of the determinant as well as the presentation. For example, a lectin might have four binding sites. In some settings, the lectin might only be able to engage two binding sites. In other settings, the lectin might bind glycans using all four of its binding sties. These could both be biologically relevant, but the avidities could be quite different. So, there are likely many “true values” for avidity for each glycan determinant. When connecting apparent Kd values measured on a glycan microarray to biological systems, I think of it as potential–“in the right context, this lectin could bind with an apparent Kd value of X to this glycan”. It has the potential to bind, but it will depend on other factors, such as the nature of the carrier chain and the spacing and orientation of the glycans.
影响因子：
15
作者：
Zhang Y;Li Q;Rodriguez LG;Gildersleeve JC
通讯作者：
Gildersleeve JC
影响因子：
3.3
作者：
Wang, Yaqi;Gildersleeve, Jeffrey C.;Basu, Amit;Zimmt, Matthew B.
通讯作者：
Zimmt, Matthew B.