Multiple regeneration pathways in Spathoglottis plicata Blume - A study in vitro
Multiple regeneration pathways in Spathoglottis plicata Blume - A study in vitro
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DOI：
10.1016/j.sajb.2012.12.005
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发表时间：
2013-03-01
影响因子：
3.1
通讯作者：
Dey, Rubel
中科院分区：
文献类型：
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作者：
Hossain, Mohammad Musharof;Dey, Rubel
本文报道了褶纹佛舌莲未成熟种子（胚）、成熟种子的非共生萌发及微繁殖。三种营养培养基，即Murashige & Skoog（MS）; Phytamax（PM）;和Phyto-Technology兰花种子播种培养基（P-723），两种碳源如2-3%（w/v）的葡萄糖和蔗糖，两种植物生长调节剂如6-苄基氨基嘌呤（6-benzylaminopurine），研究了BAP（0.5-3.0 mg l（-1））、NAA（0.5-2.0 mg l（-1））和蛋白胨（2.0 g l（-1））对种子萌发、早期原球茎发育和微繁殖的影响。成熟种子的最大发芽率（95%）记录在添加2%（w/v）蔗糖+2.0 g l（-1）蛋白胨的PM培养基中。P723 + 1.0 mg l（-1）BAP培养基对胚的萌发效果最好。从离体培养的实生苗茎段产生多个芽或类原球茎。直接器官发生和胚胎发生都被观察到，不同浓度和组合的PGRs启动了形态发生反应。最佳的PCR组合为1.0 mg l（-1）NAA +2.5 mg l（-1）BAP，1.0 mg l（-1）NAA +1.0 mg l（-1）BAP对芽的发育有促进作用，在1/2 PM +0.5 mg l（-1）IAA的培养基上诱导出粗壮的根系，将生根的小植株转移到含有由锯末、椰壳纤维、腐殖土和煤块以1：1：1：2（w/w）组成的盆栽混合物的盆中，在外部环境中存活率为80%，并在转移两年后开花。(C)2012款萨博。Elsevier B. V.出版，保留所有权利。
Asymbiotic germination of immature seeds (embryos), and mature seeds and micropropagation of spathoglottis plicata were described. Effects of three nutrition media namely, Murashige & Skoog (MS); Phytamax (PM); and Phyto-Technology orchid seed sowing medium (P-723), two carbon sources such as glucose and sucrose at 2-3% (w/v), two plant growth regulators such as 6-benzylaminopurine (BAP; 0.5-3.0 mg l(-1)) and alpha-naphthalene acetic acid (NAA; 0.5-2.0 mg l(-1)) and peptone (2.0 g l(-1)) were examined on seed germination, early protocorm development and micropropagation. The maximum germination of mature seeds (95%) was recorded in PM medium supplemented with 2% (w/v) sucrose +2.0 g l(-1) peptone. For germination of embryos P723 medium supplemented with 1.0 mg l(-1) BAP proved best. Multiple shoot buds or protocorm-like bodies (PLBs) were produced from stem segments of in vitro raised seedlings. Both direct organogenesis and embryogenesis were observed and the morphogenetic response was initiated by different concentrations and combinations of PGRs. The optimum PGR combination for maximal PLB regeneration was 1.0 mg l(-1) NAA +2.5 mg l(-1) BAP, while 1.0 mg l(-1) NAA +1.0 mg l(-1) BAP for shoot bud development Strong and stout root system was induced in half strength PM medium supplemented with 0.5 mg l(-1) IAA The well-rooted plantlets were transferred to pots containing a potting mixture composed of saw dust, coconut coir, humus, and coal pieces at 1:1:1:2 (w/w) with 80% survival in outside environment and flowered after two years of transfer. (C) 2012 SAAB. Published by Elsevier B.V. All rights reserved.