Morphological and isoenzymatic differentiation of B-chronic lymphocytic leukaemia cells induced by phorbolester.
Morphological and isoenzymatic differentiation of B-chronic lymphocytic leukaemia cells induced by phorbolester.
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佛波酯诱导的 B 慢性淋巴细胞白血病细胞的形态学和同工酶分化。
DOI：
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发表时间：
1986
影响因子：
8.8
通讯作者：
J. Minowada
中科院分区：
文献类型：
--
作者：
H. Drexler;M. Klein;N. Bhoopalam;G. Gaedicke;J. Minowada
从6例慢性B淋巴细胞白血病（CLL）患者的外周血中提取新鲜的白血病细胞，在12-O-十四烷酰佛波醇13-乙酸酯（TPA）的连续存在下进行体外诱导分化培养。TPA处理后，细胞表现出明显的形态学变化，包括细胞质和核扩大，空泡化和细胞质突起，核与核周透明带的偏心位置，以及椭圆形至细长的细胞形式。羧酸酯酶，酸性磷酸酶，氨基己糖苷酶和乳酸脱氢酶（LDH）的同工酶谱进行了分析，通过聚丙烯酰胺凝胶等电聚焦。在TPA暴露的细胞中观察到所有4种酶的同工酶的数量和染色强度增加，表明沿着B细胞途径成熟。TPA触发了抗酒石酸酸性磷酸酶同工酶（毛细胞白血病（HCL）细胞的标志物）和氨基己糖苷酶I同工酶（多发性骨髓瘤细胞的标志物）的新表达。诱导的表型变化提示分化至对应于HCL细胞或“前浆细胞”的阶段。
Fresh leukaemia cells from the peripheral blood of 6 patients with B-chronic lymphocytic leukaemia (CLL) were cultured in the continuous presence of the phorbolester 12-O-tetradecanoylphorbol 13-acetate (TPA) for in vitro induction of differentiation. Upon treatment with TPA the cells showed distinct morphological changes consisting of cytoplasmic and nuclear enlargement, vacuolisation and protrusion of cytoplasm, eccentric location of nuclei with perinuclear clear zones, and oval to elongated cell forms. Isoenzyme profiles of the enzymes carboxylic esterase, acid phosphatase, hexosaminidase and lactate dehydrogenase (LDH) were analysed by isoelectric focusing on polyacrylamide gels. An increase in the number and in the staining intensity of isoenzymes were observed for all 4 enzymes in the TPA-exposed cells indicating a maturation along the B cell pathway. TPA triggered the new expression of the tartrate-resistant acid phosphatase isoenzyme, a marker of hairy cell leukaemia (HCL) cells, and of the hexosaminidase I isoenzyme, a marker of multiple myeloma cells. The induced phenotypic changes are suggestive of differentiation to stages corresponding to those of HCL cells or 'pre-plasma cells'.
佛波酯对慢性淋巴细胞白血病细胞的表型调节：诱导 IgM 分泌和 B 细胞相关表面抗原表达的变化。
DOI：
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发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gordon,J;Mellstedt,H;Aman,P;Biberfeld,P;Klein,G
通讯作者：
Klein,G
具有小前 B 样表型的慢性淋巴细胞白血病细胞的体外分化。
影响因子：
2.7
作者：
Gordon,J;Aman,P;Mellstedt,H;Biberfeld,P;Klein,G
通讯作者：
Klein,G
毛细胞白血病：前浆细胞肿瘤。
影响因子：
20.3
作者：
Anderson,KC;Boyd,AW;Fisher,DC;Leslie,D;Schlossman,SF;Nadler,LM
通讯作者：
Nadler,LM