MOLECULAR-CLONING OF A BRAIN-SPECIFIC CALCIUM CALMODULIN-DEPENDENT PROTEIN-KINASE
MOLECULAR-CLONING OF A BRAIN-SPECIFIC CALCIUM CALMODULIN-DEPENDENT PROTEIN-KINASE
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DOI：
10.1073/pnas.84.16.5962
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发表时间：
1987-08-01
影响因子：
11.1
通讯作者：
ROSENFELD, MG
中科院分区：
文献类型：
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作者：
LIN, CR;KAPILOFF, MS;ROSENFELD, MG
钙/钙调蛋白依赖性蛋白激酶II型（CaM-K）α-已从大鼠脑中克隆了亚基cDNA。这种酶由仅在大脑中表达的5.1-脱氢酶mRNA编码。杂交组织化学显示CaM-K mRNA表达对应于免疫反应性α-CaM-K的分布。亚基蛋白，表明特定脑区的高酶水平反映了基因表达的区域差异。CaM-K α-亚基cDNA表示具有三个功能结构域的478个氨基酸（54-kDa）蛋白。域组织表明钙/钙调素依赖性和独立的状态，可能subserve短期和长期的瞬态刺激的反应的结构模型。
A calcium/calmodulin-dependent protein kinase type II (CaM-K) .alpha.-subunit cDNA has been cloned from rat brain. This enzyme is encoded by a 5.1-kilobase mRNA expressed exclusively in the brain. Hybridization histochemistry reveals that the CaM-K mRNA expression corresponds to the distribution of the immunoreactive .alpha.-subunit protein, suggesting that the high enzyme levels in specific brain areas reflect regional differences in gene expression. The sequence of CaM-K .alpha.-subunit cDNA indicates a 478-amino acid (54-kDa) protein with three functional domains. The domain organization suggests a structural model for calcium/calmodulin-dependent and independent states that might subserve short-and long-term responses to transient stimuli.