LEISHMANIA-DONOVANI - PROMASTIGOTE-MACROPHAGE SURFACE INTERACTIONS INVITRO
LEISHMANIA-DONOVANI - PROMASTIGOTE-MACROPHAGE SURFACE INTERACTIONS INVITRO
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DOI：
10.1016/0014-4894(79)90097-3
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发表时间：
1979-01-01
影响因子：
2.1
通讯作者：
CHANG, KP
中科院分区：
文献类型：
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作者：
CHANG, KP
用光学显微镜和电子显微镜对杜氏乳杆菌前鞭毛体进入金黄地鼠腹腔巨噬细胞的机制进行了研究。定量光学显微镜研究显示，细胞内寄生虫的数量随时间的增加而增加，在进入过程中没有良好的定向。扫描和透射电子显微镜显示，寄主-寄生虫的表面相互作用显著，早期在前鞭毛体周围形成螺旋状伪足，后期吞噬类似正常吞噬作用的寄生虫。扫描电子显微镜定量地将早期寄主-寄生虫相互作用分为几种类型，其中头先入和尾先入的出现频率大致相等，证实了光学显微镜的观察结果。在10微克/毫升的细胞松弛素B阻止寄生虫进入细胞内和形成巨噬细胞起源的伪足，通常被认为是抓住前鞭毛体。被杀死，但形态完好的前鞭毛虫很少被巨噬细胞吸收，并且缺乏通常与巨噬细胞伪足所遇到的某些类型的相互作用。前鞭毛体的运动性及其与巨噬细胞表面的亲和力被认为是寄生虫帮助其进入体内的重要因素。杜氏钩端螺旋体的原生质体可能依靠巨噬细胞的吞噬活性进入细胞内，但寄生虫的特异性活动和/或特性也可能起作用。这种与利什曼病-巨噬细胞相互作用相关的独特类型的内吞作用可称为促进吞噬作用。
The mechanism by which promastigotes of L. donovani enter hamster peritoneal macrophages was studied in vitro by light microscopy and EM. Quantitative light microscope studies showed a time-dependent increase of intracellular parasites, which had no preferable orientation during entry. Scanning and transmission electron microscopy revealed striking host-parasite surface interactions marked by the formation of whorled pseudopodia around the promastigotes in the early stage and engulfment of the parasites akin to normal phagocytosis in the later phase. Early host-parasite interactions were categorized quantitatively by scanning electron microscopy into several types, of which head-first entry and tail-first entry were approximately equal in frequency of occurrence, confirming light microscope observations. Cytochalasin B at 10 .mu.g/ml prevented the intracellular entry of the parasites and the formation of macrophage-originated pseudopodia normally seen to seize the promastigotes. Killed, but morphologically intact, promastigotes were poorly taken up by macrophages and lacked certain types of interactions normally encountered with macrophage pseudopodia. Motility of promastigotes and their affinity to the surface of macrophages are suggested as elements of importance which parasites contribute to aid the process of their entry. Promastigotes of L. donovani probably depend on phagocytic activity of macrophages to gain intracellular entrance, but parasite-specific activities and/or properties may also play a role. This unique type of endocytosis associated with leishmania-macrophage interactions may be termed facilitated phagocytosis.