Natural variation in CTB4a enhances rice adaptation to cold habitats.
Natural variation in CTB4a enhances rice adaptation to cold habitats.
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CTB4a 的自然变异增强了水稻对寒冷栖息地的适应
DOI：
10.1038/ncomms14788
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发表时间：
2017-03-23
影响因子：
16.6
通讯作者：
Li Z
中科院分区：
文献类型：
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作者：
Zhang Z;Li J;Pan Y;Li J;Zhou L;Shi H;Zeng Y;Guo H;Yang S;Zheng W;Yu J;Sun X;Li G;Ding Y;Ma L;Shen S;Dai L;Zhang H;Yang S;Guo Y;Li Z
低温是限制水稻产量和地理分布的主要因素。提高耐寒性并将种植范围扩大到高海拔或高纬度地区将有助于满足不断增长的大米需求。在此，我们探索了一个耐冷性QTL，并克隆了CTB 4a（孕穗期耐冷性）基因，编码一个保守的富含亮氨酸重复序列的受体样激酶。我们发现，不同的CTB 4a等位基因赋予不同水平的耐寒性和选择的CTB 4a启动子区域的变化发生在环境温度的基础上。新产生的耐冷单倍型Tej-Hap-KMXBG在温带粳稻进化过程中通过人工选择保留在寒冷的生境中进行低温驯化。此外，CTB 4a与ATP合酶的β亚基AtpB相互作用。CTB 4a的上调与增加的ATP合酶活性、ATP含量、增强的结实和在冷胁迫条件下提高的产量相关。这些发现为提高作物的耐寒性提供了策略。
Low temperature is a major factor limiting rice productivity and geographical distribution. Improved cold tolerance and expanded cultivation to high-altitude or high-latitude regions would help meet growing rice demand. Here we explored a QTL for cold tolerance and cloned the gene, CTB4a (cold tolerance at booting stage), encoding a conserved leucine-rich repeat receptor-like kinase. We show that different CTB4a alleles confer distinct levels of cold tolerance and selection for variation in the CTB4a promoter region has occurred on the basis of environmental temperature. The newly generated cold-tolerant haplotype Tej-Hap-KMXBG was retained by artificial selection during temperate japonica evolution in cold habitats for low-temperature acclimation. Moreover, CTB4a interacts with AtpB, a beta subunit of ATP synthase. Upregulation of CTB4a correlates with increased ATP synthase activity, ATP content, enhanced seed setting and improved yield under cold stress conditions. These findings suggest strategies to improve cold tolerance in crop plants.