Evaluation of laser-Doppler flowmetry as a measure of tissue blood flow.
Evaluation of laser-Doppler flowmetry as a measure of tissue blood flow.
复制标题
激光多普勒血流测量作为组织血流测量的评估。
DOI：
10.1152/jappl.1986.61.2.666
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Lombard,JH
中科院分区：
文献类型：
--
作者：
Smits,GJ;Roman,RJ;Lombard,JH
在这项研究中，通过比较麻醉大鼠肾皮质、股薄肌和提睾肌中的激光多普勒血流（LDF）信号与电磁流量计或放射性微球测量的全器官血流，评价了激光多普勒血流仪测量组织血流的技术。在体外，LDF信号密切相关（r = 0.99）的红细胞速度产生的旋转轮的变化。虽然个体LDF读数在原位变化，但从组织表面上的多个读数计算的平均LDF信号与在各种灌注压下测量的组织血流量显著相关（r = 0.74-0.95）。然而，在不同组织中以及在相同组织中使用不同测量方法时，观察到LDF信号与血流关系的斜率存在显著差异。这项研究表明，平均激光多普勒血流信号提供了一个很好的估计组织血流，提供了足够数量的点进行扫描。然而，该方法似乎没有通用的校准因子。
In this study the technique of laser-Doppler flowmetry was evaluated for the measurement of tissue blood flow by comparing laser-Doppler flow (LDF) signal in the renal cortex, gracilis muscle, and cremaster muscle of anesthetized rats to whole-organ blood flow measured with an electromagnetic flowmeter or radioactive microspheres. In vitro, LDF signal was closely correlated (r = 0.99) to changes in erythrocyte velocity generated with a rotating wheel. Although individual LDF readings varied in situ, mean LDF signal calculated from multiple readings on the tissue surface were significantly correlated (r = 0.74–0.95) with tissue blood flows measured at various perfusion pressures. However, significant differences in the slope of the LDF signal vs. blood flow relationship were observed in different tissues and with different methods of measurement in the same tissue. This study indicates that mean laser-Doppler flow signal provides a good estimate of tissue blood flow, provided a sufficient number of points is scanned. However, there appears to be no universal calibration factor for the method.