Compound stress response in stomatal closure: a mathematical model of ABA and ethylene interaction in guard cells.
Compound stress response in stomatal closure: a mathematical model of ABA and ethylene interaction in guard cells.
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DOI：
10.1186/1752-0509-6-146
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发表时间：
2012-11-25
影响因子：
--
通讯作者：
Desikan R
中科院分区：
文献类型：
--
作者：
Beguerisse-Dıaz M;Hernández-Gómez MC;Lizzul AM;Barahona M;Desikan R
气孔是植物叶片上的微小孔隙，调节植物与其环境之间的气体和水分交换。脱落酸和乙烯是两种已知的气孔关闭诱导剂。然而，当气孔与这两种信号的组合，他们无法关闭。为了阐明这种无法解释的行为，我们收集了脱落酸，乙烯和两者的组合处理的拟南芥保卫细胞的气孔孔径和过氧化氢生产的时程测量。我们的实验表明，气孔关闭与保卫细胞中持续高水平的过氧化氢有关。当用脱落酸和乙烯的组合剂量处理时，保卫细胞表现出增加的抗氧化活性，降低过氧化氢水平并阻止关闭。我们构建了一个简化的模型，气孔关闭来自已知的生化途径，捕捉实验观察到的行为。我们的实验和模拟结果表明，在气孔关闭过程中的两个抗氧化机制的不同作用：一个缓慢的，延迟的响应激活的单一刺激（脱落酸或乙烯）和另一个更快速的“和”机制，只有激活时，两种刺激都存在。我们的模型表明，在抗氧化反应中存在这种快速的“和”机制是解释在组合刺激下缺乏关闭的关键。
Stomata are tiny pores in plant leaves that regulate gas and water exchange between the plant and its environment. Abscisic acid and ethylene are two well-known elicitors of stomatal closure when acting independently. However, when stomata are presented with a combination of both signals, they fail to close. Toshed light on this unexplained behaviour, we have collected time course measurements of stomatal aperture and hydrogen peroxide production in Arabidopsis thaliana guard cells treated with abscisic acid, ethylene, and a combination of both. Our experiments show that stomatal closure is linked to sustained high levels of hydrogen peroxide in guard cells. When treated with a combined dose of abscisic acid and ethylene, guard cells exhibit increased antioxidant activity that reduces hydrogen peroxide levels and precludes closure. We construct a simplified model of stomatal closure derived from known biochemical pathways that captures the experimentally observed behaviour. Our experiments and modelling results suggest a distinct role for two antioxidant mechanisms during stomatal closure: a slower, delayed response activated by a single stimulus (abscisic acid ‘or’ ethylene) and another more rapid ‘and’ mechanism that is only activated when both stimuli are present. Our model indicates that the presence of this rapid ‘and’ mechanism in the antioxidant response is key to explain the lack of closure under a combined stimulus.
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影响因子：
3.9
作者：
Garcia-Mata, Carlos;Lamattina, Lorenzo
通讯作者：
Lamattina, Lorenzo
DOI：
10.1073/pnas.0912021106
发表时间：
2009-12-15
影响因子：
11.1
作者：
Geiger, Dietmar;Scherzer, Soenke;Hedrich, Rainer
通讯作者：
Hedrich, Rainer
DOI：
10.1073/pnas.1434381100
发表时间：
2003-09-16
影响因子：
11.1
作者：
Garcia-Mata, C;Gay, R;Blatt, MR
通讯作者：
Blatt, MR
影响因子：
7.2
作者：
Bright, J;Desikan, R;Neill, SJ
通讯作者：
Neill, SJ
影响因子：
7.4
作者：
Desikan, R;Hancock, JT;Neill, SJ
通讯作者：
Neill, SJ