Longitudinal study of molecular epidemiology of small round-structured viruses in a pediatric population
Longitudinal study of molecular epidemiology of small round-structured viruses in a pediatric population
复制标题
DOI：
10.1128/jcm.34.6.1497-1501.1996
复制
发表时间：
1996-06-01
影响因子：
9.4
通讯作者：
Petric, M
中科院分区：
文献类型：
--
作者：
Levett, PN;Gu, M;Petric, M
小圆结构病毒（SRSV），最近被指定为杯状病毒科（Caliciviridae）的成员，现在可以通过逆转录-PCR扩增其基因组的限定部分，然后通过Southern印迹用特异性探针鉴定扩增子来容易地分型，纵向调查（1991年至1995年）进行了测定散发性胃肠炎患者的小儿粪便标本中存在的SRSV基因型，结果发现，几乎所有的病毒都是G-2基因型，在探测中，亚型P2-B占优势，但不同亚型的频率每年都有变化，对1995年以来发生的SRSV群体性暴发的基因型调查表明，G-2基因型也占优势，其亚型的分布与同一时期儿科患者中散发性腹泻病例相似，结论：随着时间的推移，在我国儿童人群中存在SRSV亚型的连续性，儿童胃肠炎散发病例的基因型分布可能反映了同一时间发生的社区暴发的分布。
Small round-structured viruses (SRSV), recently designated members of the family Caliciviridae, can now be readily subtyped by amplification of a defined portion of their genome by reverse transcription-PCR and then by identification of the amplicons with specific probes by Southern blotting, A longitudinal survey (from 1991 to 1995) was conducted to determine the genotypes of the SRSV present in pediatric stool specimens from patients with sporadic cases of gastroenteritis, It was found that almost all viruses were of the G-2 genotype, and on probing, the subtype P2-B was predominant but the frequencies of the different subtypes varied from year to year, A survey of the genotypes of SRSV from community outbreaks from 1995 showed that the G-2 genotype was also predominant and that the distribution of its subtypes was similar to that seen in sporadic cases of diarrhea in pediatric patients over the same time period, It was concluded that there is a succession of subtypes of SRSV in our pediatric population over time, This distribution of genotypes in sporadic cases of pediatric gastroenteritis may reflect the distribution in community outbreaks occurring at the same time.