A proteomic analysis of salivary glands of female Anopheles gambiae mosquito
A proteomic analysis of salivary glands of female Anopheles gambiae mosquito
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DOI：
10.1002/pmic.200401210
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发表时间：
2005-09-01
期刊：
影响因子：
3.4
通讯作者：
Pandey, A
中科院分区：
文献类型：
--
作者：
Kalume, DE;Okulate, M;Pandey, A
在分子水平上了解蚊子媒介内疟原虫的发展，应该为中断寄生虫生命周期和随后的传播提供新的目标。非洲主要疟疾病媒冈比亚按蚊的完整基因组序列的可用性使得可以通过实验和计算方法发现这些目标。在雌性蚊子中，唾液腺组织在疟原虫感染形式的成熟中起着重要作用。因此，我们进行了一个蛋白质组学分析的唾液腺从女性安。冈比亚蚊子。唾液腺提取物用胰蛋白酶消化，使用两种互补的方法和LC-MS/MS分析。这导致69个独特的蛋白质，其中57个是新的鉴定。我们通过详细的生物信息学分析对本研究中鉴定的所有蛋白质进行了功能注释。尽管一些cDNA和埃德曼降解为基础的方法，目录转录本和蛋白质从蚊子的唾液腺已经出版，这是第一份报告描述的应用MS表征的唾液腺蛋白质组。我们的方法应该被证明是有价值的表征蛋白质组的寄生虫和载体的基因组测序，以及那些基因组尚未完全测序。
Understanding the development of the malaria parasite within the mosquito vector at the molecular level should provide novel targets for interrupting parasitic life cycle and subsequent transmission. Availability of the complete genomic sequence of the major African malaria vector, Anopheles gambiae, allows discovery of such targets through experimental as well as computational methods. In the female mosquito, the salivary gland tissue plays an important role in the maturation of the infective form of the malaria parasite. Therefore, we carried out a proteomic analysis of salivary glands from female An. gambiae mosquitoes. Salivary gland extracts were digested with trypsin using two complementary approaches and analyzed by LC-MS/MS. This led to identification of 69 unique proteins, 57 of which were novel. We carried out a functional annotation of all proteins identified in this study through a detailed bioinformatics analysis. Even though a number of cDNA and Edman degradation-based approaches to catalog transcripts and proteins from salivary glands of mosquitoes have been published previously, this is the first report describing the application of MS for characterization of the salivary gland proteome. Our approach should prove valuable for characterizing proteomes of parasites and vectors with sequenced genomes as well as those whose genomes are yet to be fully sequenced.