Aminated Linear and Star-Shape Poly(glycerol methacrylate)s: Synthesis and Self-Assembling Properties
Aminated Linear and Star-Shape Poly(glycerol methacrylate)s: Synthesis and Self-Assembling Properties
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DOI：
10.1021/bm901241k
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发表时间：
2010-04-01
影响因子：
6.2
通讯作者：
Leroux, Jean-Christophe
中科院分区：
文献类型：
--
作者：
Gao, Hui;Elsabahy, Mahmoud;Leroux, Jean-Christophe
在过去的10年里，聚甘油及其结构相关的类似物在生物医学领域受到了相当大的关注。聚甲基丙烯酸缩水甘油酯（PGMA）是一种多功能聚合物，因为其侧链环氧基团可以被不同的官能团打开以生成聚甲基丙烯酸甘油酯（PGOHMA）衍生物。本论文采用原子转移自由基聚合法合成了线型和星形的PGMA，然后用四种不同的胺通过开环加成进行功能化。这导致形成具有羟基和胺部分以及不同水溶性的聚甘油样聚合物。水不溶性聚合物可以形成pH敏感的纳米组装体，而可溶性衍生物有效地复合短链多核苷酸。胺化聚甘油与寡核苷酸的相互作用比对照聚（乙烯亚胺）更强烈，线性衍生物可以获得高的转染效率。这样的聚合物可以在药物和核酸的输送中找到实际应用。
Over the past 10 years, polyglycerols and their structurally related analogs have received considerable attention in the biomedical field. Poly(glycidyl methacrylate) (PGMA) is a versatile polymer because its pendant epoxide groups can be opened with different functional groups to generate poly(glycerol methacrylate)s (PGOHMA) derivatives. In this work, linear and star-shape PGMAs were synthesized by atom transfer radical polymerization and then functionalized with four different amines by ring-opening addition. This resulted in the formation of polyglycerol-like polymers having both hydroxyl and amine moieties and different water-solubility. The water-insoluble polymers could form pH-sensitive nanoassemblies, while the soluble derivatives efficiently complexed a short strand polynucleotide. The aminated polyglycerol interacted more avidly with the oligonucleotide than the control poly(ethyleneimine), and high transfection efficacy could be obtained with the linear derivative. Such polymers could find practical applications for the delivery of drugs and nucleic acids.