Aggregation-linked kinetic heterogeneity in bovine cardiac myosin subfragment 1.
Aggregation-linked kinetic heterogeneity in bovine cardiac myosin subfragment 1.
复制标题
牛心肌肌球蛋白亚片段 1 中聚集相关的动力学异质性。
DOI：
10.1021/bi00527a004
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Cusanovich,MA
中科院分区：
文献类型：
--
作者：
Flamig,DP;Cusanovich,MA
Duane P. Flamig** 和 Michael A. Cusanovich* 摘要：对 5'-三磷酸腺苷 (ATP) 结合和稳态水解速率常数的心肌肌球蛋白亚片段 1 浓度依赖性的研究表明，观察到的速率常数显着依赖于蛋白质浓度。 ATP 结合动力学是双相的，快相速率常数和慢相速率常数以及快相和慢相材料各自的分数随蛋白质浓度而变化。进行了两种不同类型的动力学实验，一种是 ATP 浓度固定，但亚片段 1 浓度变化，另一种是 ATP/亚片段 1 比例固定，但两个浓度都变化。
Duane P. Flamig** and Michael A. Cusanovich* abstract: Studies of the cardiac myosin subfragment 1 concentration dependence of the rate constants for adenosine 5'-triphosphate (ATP) binding and steady-state hydrolysis reveal that the observed rate constants are remarkably dependent on the protein concentration. The kinetics for ATP binding are biphasic, and both the fast-and slow-phase rate constants and the respective fractions of fast and slow material vary as a function of protein concentration. Two different types of kinetic experiments were conducted, one in which the ATP concentration was fixed but the subfragment 1 concen-tration was varied and another for which the ATP/subfrag-ment 1 ratio was fixed but both concentrations were varied.