Daily expression of mRNAs for the mammalian clock genes Per2 and Clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells
Daily expression of mRNAs for the mammalian clock genes Per2 and Clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells
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DOI：
10.1254/jjp.90.263
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发表时间：
2002-11-01
期刊：
影响因子：
--
通讯作者：
Shimizu, E
中科院分区：
文献类型：
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作者：
Takata, M;Burioka, N;Shimizu, E
哺乳动物的生物钟位于下丘脑的视交叉上核（SCN）和大多数外周组织中。生物钟基因驱动生物钟。然而，人类生物钟基因的昼夜表达变化仍不清楚。在这项研究中，我们分析了小鼠SCN和肝脏中mPer2和mClock mRNA表达的日常变化，以评估啮齿动物时钟基因的中枢和外周改变。我们还研究了人类外周血单核细胞（pbmc）中是否存在时钟基因hPer2和hClock的日常变化。在ZT2、ZT6、ZT10、ZT14、ZT18或ZT22时测定小鼠SCN和肝脏中mClock和mPer2 mRNA表达的日变化。我们分别于9:00和21:00从9名健康志愿者身上分离PBMCs，采用RT-PCR方法检测hPer2和hClock mRNA的表达。这些动物在SCN和肝脏中mPer2的RNA水平表现出强大的日常节律(P
The mammalian circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and in most peripheral tissues. Clock genes drive the biological clock. However, circadian expression variations of the human clock genes are still unclear. In this study, we analyzed the daily variations of mPer2 and mClock mRNA expression in both the mouse SCN and liver to evaluate the central and peripheral alterations in the rodent clock genes. We also examined whether there are the daily variations of the clock genes hPer2 and hClock in human peripheral blood mononuclear cells (PBMCs). The daily variation of mClock and mPer2 mRNA expression in mouse SCN and liver were determined at ZT2, ZT6, ZT10, ZT14, ZT18 or ZT22. We isolated PBMCs from 9 healthy volunteers at 9:00 and 21:00 and examined the expression of hPer2 and hClock mRNA by RT-PCR analysis. The animals exhibited a robust daily rhythm in the RNA levels of mPer2 in the SCN and liver (P