A novel role of Dma1 in regulating forespore membrane assembly and sporulation in fission yeast.
A novel role of Dma1 in regulating forespore membrane assembly and sporulation in fission yeast.
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Dma1 在调节裂殖酵母前孢子膜组装和孢子形成中的新作用。
DOI：
10.1091/mbc.e10-01-0079
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发表时间：
2010-12
影响因子：
3.3
通讯作者：
Jin QW
中科院分区：
文献类型：
--
作者：
Li WZ;Yu ZY;Ma PF;Wang Y;Jin QW
通过对裂殖酵母Dma 1在减数分裂过程中的功能进行分析，我们发现Dma 1是孢子形成所必需的，而它是核分裂保真度的关键。我们还发现Dma 1在孢子形成过程中与SIN途径和减数分裂特异性激酶Slk 1功能相关。在裂殖酵母裂殖酵母中，二倍体母细胞在减数分裂核分裂后通过称为孢子形成的过程分化成含有四个单倍体子囊孢子的孢子。已经鉴定出裂殖酵母的几种减数分裂特异性蛋白在减数分裂进程和孢子形成中起重要作用。我们在这里报告一个意想不到的功能，有丝分裂纺锤体检查点蛋白Dma 1在正确的孢子形成。与其在孢子形成中的功能一致，dma 1+的表达在减数分裂I和II期间上调。我们发现，Dma 1定位于SPB减数分裂过程中，这种定位在减数分裂II的维护依赖于分隔起始网络（SIN）支架蛋白Sid 4和Cdc 11。缺乏Dma 1的细胞表现出与孢子形成相关的缺陷，但不表现出核分裂，这通常导致形成孢子较少的子囊。我们的遗传分析支持Dma 1功能与减数分裂特异性Sid 2相关蛋白激酶Slk 1/Mug 27和SIN信号在孢子形成过程中平行的概念，可能通过调节适当的前孢子膜组装。因此，我们的研究揭示了一个新的功能，Dma 1在调节孢子形成的裂殖酵母。
By characterizing the fission yeast Dma1's function during meiosis, we revealed that Dma1 is required for spore formation, while it is dispensable for fidelity of nuclear divisions. We also found that Dma1 is functionally related to SIN pathway and meiosis-specific kinase Slk1 during sporulation. In fission yeast Schizosaccharomyces pombe, a diploid mother cell differentiates into an ascus containing four haploid ascospores following meiotic nuclear divisions, through a process called sporulation. Several meiosis-specific proteins of fission yeast have been identified to play essential roles in meiotic progression and sporulation. We report here an unexpected function of mitotic spindle checkpoint protein Dma1 in proper spore formation. Consistent with its function in sporulation, expression of dma1+ is up-regulated during meiosis I and II. We showed that Dma1 localizes to the SPB during meiosis and the maintenance of this localization at meiosis II depends on septation initiation network (SIN) scaffold proteins Sid4 and Cdc11. Cells lacking Dma1 display defects associated with sporulation but not nuclear division, leading frequently to formation of asci with fewer spores. Our genetic analyses support the notion that Dma1 functions in parallel with the meiosis-specific Sid2-related protein kinase Slk1/Mug27 and the SIN signaling during sporulation, possibly through regulating proper forespore membrane assembly. Our studies therefore revealed a novel function of Dma1 in regulating sporulation in fission yeast.