Analysis of differential expression proteins reveals the key pathway in response to heat stress in Alicyclobacillus acidoterrestris DSM 3922T
Analysis of differential expression proteins reveals the key pathway in response to heat stress in Alicyclobacillus acidoterrestris DSM 3922T
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差异表达蛋白分析揭示酸土脂环酸芽孢杆菌DSM 3922T响应热应激的关键途径
DOI：
10.1016/j.fm.2019.01.003
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发表时间：
2019-06-01
影响因子：
5.3
通讯作者：
Guo，Yancheng
中科院分区：
文献类型：
--
作者：
Feng，Xin;He，Chengyun;Guo，Yancheng
为了研究耐热耐酸杆菌的耐热机制，用无标记定量技术揭示了细胞内的一些变化。热应激时的酸碱度。65 °C热应激5 m in共鉴定出545个差异表达蛋白质，其中258个蛋白质表达上调，287个蛋白质表达下调。这些显著变化的蛋白质被定位在100条途径上，其中一些主要与蛋白质和DNA等大分子的保护或修复以及细胞壁的形成有关，这表明这些蛋白质可能在热胁迫反应中发挥关键作用。KEGG途径分析与蛋白质功能分析相结合，并在mRNA水平上进一步验证，表明这是一种新的表达方式。酸性物质通过改变DNA和RNA分子的二级结构来抑制环境温度的升高。抗生素的生物合成途径和核糖体可能参与了热应激的信号转导，进而触发了大量的蛋白质，在热应激的调控中起着关键作用。酸性肠杆菌。本研究首次揭示了植物对热胁迫的整体生理反应，为更好地理解植物对热胁迫的适应机制提供了依据。酸性肠杆菌。
For the purpose of investigating the heat resistance mechanism ofAlicyclobacillus acidoterrestris, label-free quantification was used to reveal some cellular changes inA. acidoterrestrisduring heat stress. Totally, 545 differential expression proteins were respectively identified at heat stress of 65 °C for 5 min, of which 258 proteins were up-regulated and 287 proteins were down-regulated. These significantly changed proteins were mapped to 100 pathways and some of them were mostly related to protection or repair of macromolecules such as proteins and DNA, cell wall formation, which indicated that these proteins might play crucial roles in response to heat stress. The KEGG pathway analysis combined with protein functional analysis and further validation at mRNA level suggested thatA. acidoterrestrissensed the temperature rise in environment through alterations in the secondary structure of DNA and RNA molecules. The biosynthesis of antibiotics pathway and the ribosomes might be involved in signal transduction in heat stress and further trigger a large number of proteins playing a critical role in the regulation of heat stress inA. acidoterrestris. The study firstly demonstrated the global physiological response to heat stress and the results provided a better understanding of thermal adaption mechanism ofA. acidoterrestris.