IDENTIFICATION OF CONTRACTILE PROTEINS IN BASAL BODIES OF CILIATED TRACHEAL EPITHELIAL-CELLS
IDENTIFICATION OF CONTRACTILE PROTEINS IN BASAL BODIES OF CILIATED TRACHEAL EPITHELIAL-CELLS
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DOI：
10.1177/28.11.7000888
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发表时间：
1980-01-01
影响因子：
3.2
通讯作者：
MILLER, F
中科院分区：
文献类型：
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作者：
GORDON, RE;LANE, BP;MILLER, F
为了确定大鼠气管上皮中纤毛细胞的基体和基体间器的组分的分子组成，使用兔抗肌动蛋白、抗α-肌动蛋白、抗α-肌动蛋白和抗α-肌动蛋白抗体。辅肌动蛋白、抗原肌球蛋白和抗肌球蛋白用作间接免疫过氧化物酶技术中应用于组织的初级抗血清。经亲和层析洗脱抗体，证明抗血清具有单特异性。偶联过氧化物酶的羊抗兔IG Fab片段用于结合的兔抗体的超微结构定位。抗α-SMA的抗体肌动蛋白围绕纤毛的外周微管，并将这些微管连接到中央双联体和质膜。α-基底足突中也有肌动蛋白。抗肌动蛋白抗体与微管的纤毛和基体，除了在该地区的纤毛项链。针对肌动蛋白的抗体还对小根、基底足突以及基底体与足突之间的通讯具有亲和力。抗肌球蛋白和抗原肌球蛋白抗体均定位于纤毛收缩区域的一部分，中央基底密度和基底体微管的外表面，以及基底足突及其与基底体的通信。这些数据表明基底体的主动收缩功能。
To determine the molecular composition of the components of basal bodies and the interbasal body apparatus of ciliated cells in rat tracheal epithelium, rabbit anti-actin, anti-.alpha.-actinin, anti-tropomyosin and anti-myosin were used as primary antisera applied to the tissue in an indirect immunoperoxidase technique. The antisera was proven to be monospecific by elution of antibody after affinity chromatography. Sheep anti-rabbit Ig Fab fragments coupled to peroxidase were used for ultrastructural localization of the bound rabbit antibody. Antibodies against .alpha.-actinin were demonstrated around peripheral microtubules of cilia and linking these microtubules to central doublet and plasma membrane. .alpha.-Actinin was also shown in the basal foot processes. Anti-actin antibodies were associated with microtubules of the cilium and basal bodies, except in the region of the ciliary necklace. The antibodies directed against actin also had affinity for rootlets, basal foot processes and communications between basal bodies and foot processes. Both anti-myosin and anti-tropomyosin antibodies were localized to part of the region of the constriction of the cilium, to the central basal density and the outer surfaces of basal body microtubules, and to the basal foot processes together with their communications to the basal body. The data suggest active contractile function of basal bodies.